From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • pan locus tag?: SAUPAN006253000
  • symbol: SA2365
  • pan gene symbol?: gbaB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • symbol: SA2365
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2655134..2655838
  • length: 705
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACTAAAATTGTGTTAATCACAGGAGGCAATAAAGGGTTAGGCTATGCAAGTGCAGAG
    GCACTTAAAGCATTGGGTTACAAAGTTTATATAGGTTCTCGGAATGATGTAAGAGGACAA
    CAAGCATCACAAAAATTAGGTGTTCATTATGTACAGTTAGATGTTACAAGTGACTACTCA
    GTCAAAAATGCTTATAACATGATTGCTGAAAAAGAAGGCCGTCTTGATATCCTCATTAAC
    AATGCAGGTATATCTGGTCAATTTTCAGCACCTTCCAAATTGACACCTCGTGACGTTGAA
    GAAGTATATCAAACGAATGTATTCGGTATCGTACGAATGATGAATACATTCGTCCCTCTC
    TTAGAAAAATCTGAACAACCTGTTGTCGTCAACGTATCAAGTGGTTTAGGTTCATTTGGA
    ATGGTTACAAACCCCGAAACAGCTGAATCTAAAGTGAATTCATTAGCTTATTGTTCGTCT
    AAATCGGCAGTAACAATGCTAACGCTACAATACGCTAAAGGATTACCTAACATGCAAATC
    AATGCTGCTGACCCAGGCGCTACAAATACTGATTTAGTTGGTGATTTTAGTAATAATTCC
    AAACACGTTTCTGAAGGAATCAAGCCAATCATTCAATTAGCAACGATTGGTGCAGATGGC
    CCAACAGGTACATTTATTAATGGCGATGGAGAAATGCCTTGGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2365 [new locus tag: SA_RS13550 ]
  • symbol: SA2365
  • description: hypothetical protein
  • length: 234
  • theoretical pI: 5.80622
  • theoretical MW: 24931.1
  • GRAVY: -0.126923

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 79.9)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 79)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 69)
    Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 64.8)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 64.1)
    and 12 more
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 62.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 58.1)
    cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 49.1)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 47.3)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 42.7)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 36.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 34.4)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 33.3)
    sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.4)
    pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 16)
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 13.5)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 12.1)
  • TheSEED  :
    • Oxidoreductase, short-chain dehydrogenase/reductase family
    Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 135.2)
    and 7 more
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 90.8)
    KR; KR domain (PF08659; HMM-score: 35.2)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 21.9)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 17.6)
    SDR; SDR-like rossmann domain (PF23441; HMM-score: 16.5)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 14)
    PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.965
    • Cytoplasmic Membrane Score: 0.0029
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0318
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030131
    • TAT(Tat/SPI): 0.001482
    • LIPO(Sec/SPII): 0.004751
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQKLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEVYQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSSKSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGADGPTGTFINGDGEMPW

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA2365&oldid=77974"