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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2260 [new locus tag: SA_RS12975 ]
  • pan locus tag?: SAUPAN006035000
  • symbol: SA2260
  • pan gene symbol?: —
  • synonym:
  • product: short chain dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2260 [new locus tag: SA_RS12975 ]
  • symbol: SA2260
  • product: short chain dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2537697..2538515
  • length: 819
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAACGTTTGGAAAATAAAGTTGCAGTCGTAACAGGAGCAAGTACAGGTATCGGACAA
    GCTTCTGCAATCGCTTTAGCTCAAGAAGGTGCGTATGTATTGGCGGTAGACATAGCTGAA
    GCGGTATCGGAGACTGTCGATAAAATTAAAAGTAACGGTGACAATGCGAAGGCGTATAAT
    GTGGATATTTCAGATGAACAACAAGTGGTAGACTTTGTGTCTGACATAAAGGAACAGTTT
    GGAAGAATCGATGTGTTGTTTAATAATGCCGGTGTGGATAATGCGGCTGGTAGAATTCAT
    GAGTATCCAATAGATGTGTATGACAAGATTATGAATGTAGATATGCGTGGGACAATTTTA
    ATGACGAAAATGATGTTACCTTTAATGATGAATCAAGGTGGCTCTATTGTTAATACGTCT
    TCATTTTCAGGACAGGCAGAAGATTTGTATCGCTCAGGATATAATGCTGCGAAAGGCGCA
    GTGATTAATTTTACAAAATCAATCGCAATTGAGTATGGTCGTGATGGCATTCGATCCAAT
    GCAATTGCACCAGGTACAATTGAAACCCCGTTAGTAGATAAACTGACAGGTACGAGTGAG
    GATGATGCAGGTAAAACATTTAGAGAAAATCAAAAATGGATGACTCCGCTGGGACGTTTA
    GGTAAACCAGAAGAAGTTGCTAAATTAGTAGTCTTCTTAGCATCTGACGACAGTTCATTC
    ATAACTGGAGAGACGATTCGAATTGATGGTGGTGTGATGGCTTACACATGGCCTGGCGAG
    ATGTTAAGTGATGATTCGTGGAAGCGGACATTGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2260 [new locus tag: SA_RS12975 ]
  • symbol: SA2260
  • description: short chain dehydrogenase
  • length: 272
  • theoretical pI: 4.40492
  • theoretical MW: 29453.1
  • GRAVY: -0.191912

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 194.9)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 191.8)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 183.7)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 170)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 165)
    Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 164.1)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 162.8)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 156.3)
    and 8 more
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 128.3)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 113.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 111.4)
    cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 87.9)
    pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 70.2)
    sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 39.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 33.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 16.9)
  • TheSEED  :
    • Oxidoreductase, short-chain dehydrogenase/reductase family
  • ⊞PFAM:
    NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 228.9)
    and 9 more
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 179)
    KR; KR domain (PF08659; HMM-score: 46.4)
    SDR; SDR-like rossmann domain (PF23441; HMM-score: 31.8)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 21.1)
    Eno-Rase_NADH_b; NAD(P)H binding domain of trans-2-enoyl-CoA reductase (PF12242; HMM-score: 15.8)
    GBD (CL0202) HpiC1-like; HpiC1 cyclase (PF22825; HMM-score: 15.7)
    PH (CL0266) OCRL_clath_bd; Inositol polyphosphate 5-phosphatase clathrin binding domain (PF16726; HMM-score: 14.3)
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 13.6)
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.5
    • Cellwall Score: 4.07
    • Extracellular Score: 5.43
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9494
    • Cytoplasmic Membrane Score: 0.0133
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.037
  • ⊞LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: SAIALAQE
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.698318
    • TAT(Tat/SPI): 0.035546
    • LIPO(Sec/SPII): 0.00796
    • Cleavage Site: CS pos: 27-28. ALA-QE. Pr: 0.4279
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSETVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEYPIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSGQAEDLYRSGYNAAKGAVINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLGRLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CodY (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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