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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1940 [new locus tag: SA_RS11145 ]
  • pan locus tag?: SAUPAN005435000
  • symbol: deoD
  • pan gene symbol?: deoD
  • synonym:
  • product: purine nucleoside phosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1940 [new locus tag: SA_RS11145 ]
  • symbol: deoD
  • product: purine nucleoside phosphorylase
  • replicon: chromosome
  • strand: +
  • coordinates: 2189902..2190612
  • length: 711
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACAAAAGGTACACCACATATTCAACCAAATGGAGTAAAAATTGCTAAAACAGTATTA
    ATGCCTGGCGATCCGCTACGCGCAAAATATATTGCTGATAATTTTTTAGAAAATGTTGAA
    CAATTTAACGATGTACGTAACATGTTTGGTTACACTGGTACATATAAAGGTAAAGAAGTT
    TCTGTAATGGGTTCTGGTATGGGTATTCCAAGTATTGGTATTTACTCATATGAGTTATAC
    AACTTCTTTGATGTAGATACAATCATTCGTATCGGTTCTTGTGGCGCATTACAAGAAAAT
    GTTAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGAT
    CAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCT
    AAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGAT
    ACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGT
    ATCGAAATGGAATCAGCTGGTTTATATTTAAATGCGATTCATGCTGGTAAAAAAGCACTT
    GGTATTTTCACAGTAAGTGATCATATTTTACGTGACGAAGCTACTACACCTGAAGAACGT
    CAAAATTCATTTACACAAATGATGGAAATCGCTTTAGAAATCGCAGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    711

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1940 [new locus tag: SA_RS11145 ]
  • symbol: DeoD
  • description: purine nucleoside phosphorylase
  • length: 236
  • theoretical pI: 4.57428
  • theoretical MW: 25908.1
  • GRAVY: -0.12839

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.4.2.1?  ExPASy
    Purine-nucleoside phosphorylase Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides purine nucleoside phosphorylase (TIGR00107; EC 2.4.2.1; HMM-score: 309.6)
    and 7 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine phosphorylase (TIGR01718; EC 2.4.2.3; HMM-score: 120.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides MTA/SAH nucleosidase (TIGR01704; EC 3.2.2.16,3.2.2.9; HMM-score: 37)
    Metabolism Central intermediary metabolism Other MTA/SAH nucleosidase (TIGR01704; EC 3.2.2.16,3.2.2.9; HMM-score: 37)
    putative AMP nucleosidase (TIGR01721; HMM-score: 29.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine phosphorylase (TIGR01719; EC 2.4.2.3; HMM-score: 26.3)
    futalosine hydrolase (TIGR03664; EC 3.2.2.26; HMM-score: 24)
    hopanoid-associated phosphorylase (TIGR03468; HMM-score: 22.4)
  • ⊞TheSEED  :
    • Purine nucleoside phosphorylase (EC 2.4.2.1)
    Carbohydrates Monosaccharides Deoxyribose and Deoxynucleoside Catabolism  Purine nucleoside phosphorylase (EC 2.4.2.1)
    and 2 more
    Nucleosides and Nucleotides Purines Purine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Purine nucleoside phosphorylase (EC 2.4.2.1)
  • PFAM:
    PUP (CL0408) PNP_UDP_1; Phosphorylase superfamily (PF01048; HMM-score: 136.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9869
    • Cytoplasmic Membrane Score: 0.0013
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0118
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013157
    • TAT(Tat/SPI): 0.000503
    • LIPO(Sec/SPII): 0.000946
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFNDVRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVNLYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSDTFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPEERQNSFTQMMEIALEIAE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • ⊟interaction partners:
    SA0627hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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