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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0419 [new locus tag: SA_RS02390 ]
  • pan locus tag?: SAUPAN002175000
  • symbol: metB
  • pan gene symbol?: mccB
  • synonym:
  • product: cystathionine gamma-synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0419 [new locus tag: SA_RS02390 ]
  • symbol: metB
  • product: cystathionine gamma-synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 480605..481747
  • length: 1143
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATAAGAAAACTAAATTAATTCACGGTGGACACACAACGGACGACTATACAGGTGCA
    GTTACTACACCAATTTATCAAACAAGTACATATTTACAAGATGATATTGGTGATTTACGT
    CAAGGATATGAATATTCTCGTACTGCGAATCCAACAAGAAGTTCTGTAGAAAGCGTTATT
    GCGGCATTAGAAAATGGCAAACATGGCTTTGCGTTTAGTTCAGGTGTTGCAGCTATCAGT
    GCAGTTGTTATGCTGTTGGACAAAGGTGATCATATTATTTTAAATTCAGATGTATACGGC
    GGTACTTATCGCGCATTGACAAAAGTATTTACACGATTTGGCATTGAAGTGGATTTTGTA
    GATACAACGCATACAGATTCAATTGTACAAGCGATACGCCCAACAACAAAGATGTTGTTT
    ATTGAAACACCTTCTAATCCATTATTACGTGTTACTGACATTAAAAAGTCTGCTGAAATT
    GCGAAAGAACACGGTTTGATTTCAGTCGTTGATAACACATTTATGACACCTTATTATCAG
    AATCCATTAGATTTAGGCATCGATATTGTCTTGCATTCTGCAACGAAATATTTAGGTGGA
    CATAGTGATGTCGTTGCTGGTTTAGTTGCAACATCGGATGACAAGCTTGCAGAACGTTTA
    GCATTTATTTCAAATTCAACAGGTGGCATTTTAGGACCTCAAGATAGCTATTTACTTGTG
    AGGGGTATTAAAACATTAGGTTTACGTATGGAACAAATTAATCGCAGTGTTATTGAAATT
    ATTAAAATGTTACAAGCACATCCAGCTGTGCAACAAGTGTTCCATCCAAGTATTGAAAGT
    CATTTAAATCATGATGTCCATATGGCTCAAGCGGATGGCCATACAGGTGTGATTGCATTT
    GAAGTGAAAAATACAGAAAGTGCCAAACAATTGATTAAAGCAACATCGTATTACACATTA
    GCTGAAAGTTTAGGTGCAGTGGAAAGTTTAATTTCAGTACCTGCATTGATGACACATGCA
    TCCATTCCAGCAGATATTCGAGCTAAAGAAGGTATTACAGACGGACTTGTAAGAATTTCT
    GTAGGTATTGAAGATACTGAAGATTTAGTCGATGATTTAAAACAAGCACTAGATACGCTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0419 [new locus tag: SA_RS02390 ]
  • symbol: MetB
  • description: cystathionine gamma-synthase
  • length: 380
  • theoretical pI: 5.67217
  • theoretical MW: 41248.5
  • GRAVY: -0.0371053

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 398.1)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 392.1)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 379.3)
    and 16 more
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 315.5)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 315.5)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 313.9)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 239.5)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 32.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 22.9)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 21.6)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 21.2)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 17.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 17.2)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 16.7)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.8)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 13.3)
    enduracididine biosynthesis enzyme MppP (TIGR04462; EC 2.-.-.-; HMM-score: 13.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 13)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 11)
  • ⊞TheSEED  :
    • Cystathionine gamma-lyase (EC 4.4.1.1)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Cystathionine gamma-lyase (EC 4.4.1.1)
    and 3 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis  Cystathionine gamma-lyase (EC 4.4.1.1)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Cystathionine gamma-lyase (EC 4.4.1.1)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Cystathionine gamma-lyase (EC 4.4.1.1)
  • ⊞PFAM:
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 497.7)
    and 5 more
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 29.9)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 27.3)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 19.2)
    no clan defined IF-2; Translation-initiation factor 2 (PF11987; HMM-score: 13.2)
    PDDEXK (CL0236) PDDEXK_13; PD-(D/E)XK nuclease superfamily (PF20796; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9876
    • Cytoplasmic Membrane Score: 0.0013
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.011
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021337
    • TAT(Tat/SPI): 0.00093
    • LIPO(Sec/SPII): 0.002107
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: CymR* (repression) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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