⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS01005 [old locus tag: SACOL0197 ]
pan locus tag^?: SAUPAN001070000
symbol: SACOL_RS01005
pan gene symbol^?: —
• synonym:
• product: gfo/Idh/MocA family oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01005 [old locus tag: SACOL0197 ]
• symbol: SACOL_RS01005
• product: gfo/Idh/MocA family oxidoreductase
• replicon: chromosome
• strand: +
• coordinates: 231072..232112
• length: 1041
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (231072..232112) NCBI
• BioCyc: SACOL_RS01005 BioCyc
• MicrobesOnline: see SACOL0197

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  
  ATGACAAAATTAAAAGTTGGTGTGATAGGTGTTGGTGGTATTGCACAAGACCGTCATATT
  CCAGCATTGCTGAAACTCAAAGACACAGTCTCATTAGTTGCAGTACAAGATATTAATACA
  GTGCAGATGATTGATGTTGCGAAGCGCTTTAATATACCTCATGCAGTTGAGACACCTAGC
  GAGCTGTTTAAACTTGTTGATGCGGTGGTCATTTGTACACCTAATAAATTCCATGCTGAT
  CTTTCTATAGAAGCATTGAACCATGGTGTCCATGTATTGTGTGAAAAGCCAATGGCGATG
  ACGACGGAAGAGTGTGATCGCATGATTGAAGCGGCTAATAAAAATCACAAATTATTAACT
  GTCGCATATCATTATCGTCACACAGATGTGGCAATTACTGCTAAAAAAGCAATTGAATCA
  GGTGTGGTTGGTAAACCTTTAGTAGCACGTGTACAAGCGATGCGTAGGCGTAAAGTGCCT
  GGCTGGGGTGTTTTTACCAATAAAGCGTTGCAAGGTGGCGGTAGTTTAATCGATTATGGT
  TGCCACTTGTTAGACTTATCTTTGTGGCTACTAGGTAAAGATATGGTGCCGCATGAAGTG
  CTAGGAAAAACATATAATCAATTGAGCAAACAACCGAATCAAATTAATGATTGGGGAACA
  TTTGATCATACTAAATTTGATGTCGATGATCATGTTACTAGTTATATGACATTTGCCAAT
  CGAGCAAGCATGCAGTTTGAATGTTCGTGGTCTGCAAATATCAAAGAAGATAAGGTTCAC
  GTTAGTTTATCAGGAGAAGATGGCGGTATCAATTTATTTCCATTTGAAATATATGAGCCC
  CGCTTTGGAACTATTTTTGAAAGCAAAGCTAATGTTGAGCATAACGAAGACATTGCTGGT
  GAGAGACAGGCGCGTAACTTTGTCAATGCGTGTTTAGGGATAGAAGAGATTGTGGTGAAA
  CCGGAAGAAGCACGCAATGTAAATGCCCTTATAGAAGCGATTTATCGTAGCGATCTTGAT
  AACAAGAGCATACAACTTTAA
  60
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  1041
  

⊟Protein

⊟General

• locus tag: SACOL_RS01005 [old locus tag: SACOL0197 ]
• symbol: SACOL_RS01005
• description: gfo/Idh/MocA family oxidoreductase
• length: 346
• theoretical pI: 6.62675
• theoretical MW: 38557
• GRAVY: -0.167919

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Sugars inositol 2-dehydrogenase (TIGR04380; EC 1.1.1.18; HMM-score: 105.2)
• TheSEED: see SACOL0197
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 109.6)
  and 5 more
  GADPH_aa-bio_dh (CL0139) GFO_IDH_MocA_C3; GFO/IDH/MocA C-terminal domain (PF22725; HMM-score: 76.9)
  GFO_IDH_MocA_C; Oxidoreductase family, C-terminal alpha/beta domain (PF02894; HMM-score: 63)
  NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 21.7)
  CoA_binding; CoA binding domain (PF02629; HMM-score: 14.6)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9839
  • Cytoplasmic Membrane Score: 0.009
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0069
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006148
  • TAT(Tat/SPI): 0.000207
  • LIPO(Sec/SPII): 0.0011
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446085954 NCBI
• RefSeq: WP_000163809 NCBI
• UniProt: see SACOL0197

⊟Protein sequence

• MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMIDVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTTEECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVPGWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDWGTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFEIYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAIYRSDLDNKSIQL

⊟Experimental data

• experimentally validated: see SACOL0197
• protein localization: see SACOL0197
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: MalR see SACOL0197

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications