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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0486 [new locus tag: SA_RS02860 ]
  • pan locus tag?: SAUPAN002294000
  • symbol: gltX
  • pan gene symbol?: gltX
  • synonym:
  • product: glutamyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0486 [new locus tag: SA_RS02860 ]
  • symbol: gltX
  • product: glutamyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 568646..570100
  • length: 1455
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGTTATCTTCATATTGGT
    AATGCAAGAACAGCATTATTCAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTG
    ATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAGATGGAGAAACATCACAATTT
    GATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
    GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTA
    GCAGAAGATAAAGCATATAAATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAA
    GCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTCAACATGCGCATTTGACTGAA
    GAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
    CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAAT
    GGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTA
    GCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCA
    AACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
    CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATT
    TTACAATTTATTGAGCAATATCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTT
    ATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAATCTTTTCTAAAGAAGAATTT
    ATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
    TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTA
    GCATTACCTCATTTAATTAAAGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTA
    TCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAATGAGTTATGCCGGTGAAATT
    GTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
    GTGATTAATGGAGAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCA
    CTTGAACCATTTGAAGCGGCTGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACA
    GGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTGCTGTAACAGGCCAAATGCAT
    GGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
    AAACAATATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0486 [new locus tag: SA_RS02860 ]
  • symbol: GltX
  • description: glutamyl-tRNA synthetase
  • length: 484
  • theoretical pI: 4.97567
  • theoretical MW: 56288.4
  • GRAVY: -0.639669

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.1.1.17?  ExPASy
    Glutamate--tRNA ligase ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation glutamate--tRNA ligase (TIGR00464; EC 6.1.1.17; HMM-score: 624.4)
    and 4 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification glutamyl-queuosine tRNA(Asp) synthetase (TIGR03838; EC 6.1.1.-; HMM-score: 219.3)
    Genetic information processing Protein synthesis tRNA aminoacylation glutamate--tRNA ligase (TIGR00463; EC 6.1.1.17; HMM-score: 127.7)
    Genetic information processing Protein synthesis tRNA aminoacylation glutamine--tRNA ligase (TIGR00440; EC 6.1.1.18; HMM-score: 52.3)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 28.1)
  • ⊞TheSEED  :
    • tRNA glutamyl-Q(34)-Gln synthetase (EC 6.1.1.24)
    • tRNA glutamyl-Q(34)-Glu synthetase (EC 6.1.1.17)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Glutamyl-tRNA synthetase (EC 6.1.1.17)
    and 3 more
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  tRNA glutamyl-Q(34)-Gln synthetase (EC 6.1.1.24)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln  tRNA glutamyl-Q(34)-Glu synthetase (EC 6.1.1.17)
  • ⊞PFAM:
    HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 412.1)
    and 4 more
    no clan defined Anticodon_2; Anticodon binding domain (PF19269; HMM-score: 105.2)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.1)
    HTH (CL0123) Mrr_N; Mrr N-terminal domain (PF14338; HMM-score: 13.3)
    no clan defined VMAP-M5; vWA-MoxR associated protein middle region (VMAP-M) 5 (PF19967; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9996
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003965
    • TAT(Tat/SPI): 0.000323
    • LIPO(Sec/SPII): 0.000767
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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