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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03800 [old locus tag: SACOL0740 ]
  • pan locus tag?: SAUPAN002564000
  • symbol: SACOL_RS03800
  • pan gene symbol?: —
  • synonym:
  • product: Rossman fold protein, TIGR00730 family

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03800 [old locus tag: SACOL0740 ]
  • symbol: SACOL_RS03800
  • product: Rossman fold protein, TIGR00730 family
  • replicon: chromosome
  • strand: -
  • coordinates: 761463..762029
  • length: 567
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (761463..762029) NCBI
  • BioCyc: SACOL_RS03800 BioCyc
  • MicrobesOnline: see SACOL0740

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAACGAATCGCAGTTTATTGTGGTGCAAGCAAAGGTCATGACCCTTCATATGTACAA
    AAAGCATACGATTTAGGTAAATATTTTGCTGAACAAGGTTATGAATTAGTATTTGGTGCA
    GGGTCAATTGGCATTATGGGTGCCATTCAAGATGGTGTATTAGATCATGGCGGCAAAGCT
    ATTGGCGTCATGCCTAAAATGTTAGATGAACATGAAATCACAAGCCAACGTTTAACTGAA
    TTAATTTTAGTTGATTCTATGCATGAACGTAAAAATAAAATGGCAGAACTTGCAGATGCA
    TTTGTCATGGCTCCTGGTGGCGCAGGTTCACTAGAAGAATTTTTCGAAATGTATAGTTGG
    GCTCAAATAGGTATCCATGAAAAGCCTATTGCCATTTATAATATTAATGGCTTTTTCAAT
    CCACTACAAACAATGATTGACCATATGATTGAGGAAGGTTTTATTGATCCTAAGTATCGT
    GCTCTTGCACCGTTATGCGACACTAAGGAATCATTAATTGAATCAATATTAAATTTCAAA
    CCTCTGGGTACTCGTTCATACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03800 [old locus tag: SACOL0740 ]
  • symbol: SACOL_RS03800
  • description: Rossman fold protein, TIGR00730 family
  • length: 188
  • theoretical pI: 4.89275
  • theoretical MW: 20888.9
  • GRAVY: -0.114894

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 3.2.2.n1?  ExPASy
    Cytokinin riboside 5'-monophosphate phosphoribohydrolase N6-(Delta2-isopentenyl)-adenosine 5'-phosphate + H2O = N6-(dimethylallyl)adenine + D-ribose 5'-phosphate Trans-zeatin riboside phosphate + H2O = trans-zeatin + D-ribose 5'-phosphate
  • ⊞TIGRFAM:
    Hypothetical proteins Conserved TIGR00730 family protein (TIGR00730; HMM-score: 197.5)
    and 3 more
    Hypothetical proteins Conserved TIGR00725 family protein (TIGR00725; HMM-score: 37.6)
    Cellular processes Cellular processes DNA transformation DNA protecting protein DprA (TIGR00732; HMM-score: 13)
    gliding motility-associated lipoprotein GldK (TIGR03525; HMM-score: 12.6)
  • TheSEED: see SACOL0740
  • ⊞PFAM:
    SLOG (CL0349) Lysine_decarbox; Possible lysine decarboxylase (PF03641; HMM-score: 165.3)
    and 1 more
    LDcluster4; SLOG cluster4 family (PF18306; HMM-score: 58)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9912
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0085
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017898
    • TAT(Tat/SPI): 0.000237
    • LIPO(Sec/SPII): 0.000832
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446743636 NCBI
  • RefSeq: WP_000820892 NCBI
  • UniProt: see SACOL0740

⊟Protein sequence[edit | edit source]

  • MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIGIMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFVMAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYRALAPLCDTKESLIESILNFKPLGTRSYD

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0740
  • protein localization: see SACOL0740
  • quantitative data / protein copy number per cell: see SACOL0740
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* see SACOL0740

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS03800&oldid=97574"
  • This page was last edited on 11 March 2016, at 11:38.
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