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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0519 [new locus tag: SACOL_RS02630 ]
  • pan locus tag?: SAUPAN002197000
  • symbol: SACOL0519
  • pan gene symbol?: —
  • synonym:
  • product: acetyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0519 [new locus tag: SACOL_RS02630 ]
  • symbol: SACOL0519
  • product: acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 524993..525517
  • length: 525
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3237094 NCBI
  • RefSeq: YP_185407 NCBI
  • BioCyc: see SACOL_RS02630
  • MicrobesOnline: 911988 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCAAATATATTTAAGTACTTTAACAGAGTTAGATTATGATAAATCTTTAAATAGTATT
    GAAGAAAGTTTTGATGATAATCCTGAAACGAGTTGGCAAGCACGTGCGAAAGTAAAACAT
    TTAAGAAAATCTCCTTGCTATAATTTTGAATTAGAAGTAATAGCGAAAAATGAAAATAAC
    GATGTCGTTGGACACGTTTTATTAATTGAAGTAGAAATTAATAGTGATGATAAGACGTAT
    TATGGTTTGGCGATTGCCTCTTTATCAGTTCATCCTGAATTACGTGGACAAAAATTAGGT
    CGTGGCTTGGTTCAAGCAGTAGAAGAGCGTGCCAAAGCACAAGAGTATAGTACGGTTGTT
    GTAGACCATTGTTTTGACTACTTTGAAAAGTTGGGTTATCAAAATGCTGCTGAGCATGAC
    ATTAAATTAGAATTTGGTGATGCACCGTTACTTGTAAAATATTTATGGGATAATTTGACG
    GATGCACCACACGGAATCGTAAAATTTCCAGAACATTTTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    525

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0519 [new locus tag: SACOL_RS02630 ]
  • symbol: SACOL0519
  • description: acetyltransferase
  • length: 174
  • theoretical pI: 4.62169
  • theoretical MW: 19968.2
  • GRAVY: -0.424713

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 18.8)
    Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 15.1)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 13)
  • TheSEED  :
    • Acetyltransferase (GNAT) family protein
  • ⊞PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 39.5)
    Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 35.2)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 34.6)
    and 4 more
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 27.9)
    NMT; Myristoyl-CoA:protein N-myristoyltransferase, N-terminal domain (PF01233; HMM-score: 19)
    Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 17.6)
    FR47; FR47-like protein (PF08445; HMM-score: 17.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9658
    • Cytoplasmic Membrane Score: 0.006
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0278
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008309
    • TAT(Tat/SPI): 0.000829
    • LIPO(Sec/SPII): 0.001064
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 57651358 NCBI
  • RefSeq: YP_185407 NCBI
  • UniProt: A0A0H2WYH8 UniProt

⊟Protein sequence[edit | edit source]

  • MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKSPCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRGLVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLEFGDAPLLVKYLWDNLTDAPHGIVKFPEHFY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0519&oldid=90496"
  • This page was last edited on 11 March 2016, at 06:56.
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