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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS14295 [old locus tag: SACOL2735 ]
  • pan locus tag?: SAUPAN006486000
  • symbol: SACOL_RS14295
  • pan gene symbol?: noc
  • synonym:
  • product: nucleoid occlusion protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS14295 [old locus tag: SACOL2735 ]
  • symbol: SACOL_RS14295
  • product: nucleoid occlusion protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2803561..2804400
  • length: 840
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2803561..2804400) NCBI
  • BioCyc: SACOL_RS14295 BioCyc
  • MicrobesOnline: see SACOL2735

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
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    481
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    601
    661
    721
    781
    ATGAAAAAACCTTTTTCAAAATTATTTGGTTTGAAAAACAAAGATGACATCATTGGACAT
    ATTGAAGAAGATCGCAATAGTAATGTTGAATCCATTCAAATTGAACGTATCGTTCCCAAC
    CGTTATCAACCAAGACAGGTGTTTGAACCAAATAAAATTAAAGAACTTGCTGAATCAATA
    CATGAACATGGTTTACTACAACCTATTGTTGTAAGACCGATTGAAGAAGATATGTTTGAA
    ATTATTGCTGGAGAGCGCCGATTTAGAGCAATACAATCACTAAATTTACCTCAAGCAGAC
    GTTATTATTCGTGATATGGATGATGAAGAGACGGCTGTTGTTGCATTAATTGAGAATATT
    CAAAGAGAAAATTTGTCTGTTGTTGAAGAAGCGGAAGCCTATAAGAAATTATTGGAAATT
    GGTGATACAACGCAAAGTGAATTGGCAAAAAGTTTAGGTAAAAGTCAAAGCTTTATTGCA
    AATAAGTTGCGTTTATTGAAGTTGGCGCCGAAAGTACTACTTCGCTTAAGAGAAGGTAAA
    ATTACTGAACGTCATGCGAGAGCGGTATTATCATTGTCTGATAGCGAACAAGAAGCGTTG
    ATTGAGCAAGTCATTGCACAAAAGCTAAATGTGAAACAGACTGAAGATAGAGTACGCCAA
    AAAACGGGGCCCGAAAAAGTCAAAGCACAAAACCTTCGCTTTGCACAAGATGTCACTCAA
    GCACGAGATGAGGTAGGCAAAAGTATCCAAGCGATTCAACAAACAGGATTACATGTTGAG
    CATAAAGACAAAGATCATGAAGATTATTATGAAATAAAAATTCGAATATATAAACGTTAG
    60
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⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS14295 [old locus tag: SACOL2735 ]
  • symbol: SACOL_RS14295
  • description: nucleoid occlusion protein
  • length: 279
  • theoretical pI: 6.90987
  • theoretical MW: 32196.6
  • GRAVY: -0.620072

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    nucleoid occlusion protein (TIGR04285; HMM-score: 318)
    and 4 more
    ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 185.8)
    PRTRC system ParB family protein (TIGR03734; HMM-score: 140.9)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepB (TIGR03454; HMM-score: 61.6)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.8)
  • TheSEED: see SACOL2735
  • ⊞PFAM:
    ParBc (CL0248) ParBc; ParB/Sulfiredoxin domain (PF02195; HMM-score: 98.5)
    and 5 more
    HTH (CL0123) HTH_ParB; HTH domain found in ParB protein (PF17762; HMM-score: 61.3)
    KorB; KorB domain (PF08535; HMM-score: 19.1)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 17)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 14.9)
    HTH_3; Helix-turn-helix (PF01381; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9915
    • Cytoplasmic Membrane Score: 0.0025
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0046
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006174
    • TAT(Tat/SPI): 0.000314
    • LIPO(Sec/SPII): 0.000923
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446665491 NCBI
  • RefSeq: WP_000742837 NCBI
  • UniProt: see SACOL2735

⊟Protein sequence[edit | edit source]

  • MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQPRQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVIIRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIANKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRVRQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRIYKR

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2735
  • protein localization: see SACOL2735
  • quantitative data / protein copy number per cell: see SACOL2735
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS14295&oldid=93169"
  • This page was last edited on 11 March 2016, at 09:19.
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