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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13280 [old locus tag: SACOL2535 ]
  • pan locus tag?: SAUPAN006169000
  • symbol: SACOL_RS13280
  • pan gene symbol?: ddh
  • synonym:
  • product: lactate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS13280 [old locus tag: SACOL2535 ]
  • symbol: SACOL_RS13280
  • product: lactate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2596114..2597106
  • length: 993
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2596114..2597106) NCBI
  • BioCyc: SACOL_RS13280 BioCyc
  • MicrobesOnline: see SACOL2535

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAAAAATTATGTTCTTTGGTACGCGTGATTATGAGAAAGAGATGGCATTAAATTGG
    GGGAAAAAGAATAATGTCGAAGTAACTACTTCTAAAGAGCTATTATCAAGTGCTACAGTC
    GATCAATTAAAAGATTACGATGGCGTAACTACAATGCAATTTGGTAAGTTAGAAAATGAC
    GTTTATCCTAAATTAGAATCTTACGGTATTAAACAAATTGCACAACGTACTGCTGGATTT
    GATATGTATGATTTAGATTTAGCTAAAAAACACAATATTGTGATATCTAACGTTCCTAGT
    TATTCACCTGAAACAATTGCAGAGTATTCTGTTTCTATCGCCCTACAATTAGTGCGTCGC
    TTCCCAGATATTGAACGCCGTGTACAAGCACATGATTTTACTTGGCAAGCAGAAATCATG
    TCTAAACCAGTTAAAAATATGACTGTTGCAATTATTGGTACGGGTCGTATCGGTGCTGCT
    ACAGCTAAAATATATGCAGGATTTGGTGCTACAATTACAGCTTATGACGCCTATCCTAAT
    AAAGATTTAGACTTTTTAACTTATAAAGATAGTGTTAAAGAAGCTATTAAAGATGCCGAT
    ATTATTTCTTTACATGTTCCAGCGAACAAAGAAAGCTATCATTTATTCGATAAAGCAATG
    TTTGATCATGTGAAAAAAGGTGCAATCTTAGTTAACGCAGCACGTGGTGCAGTCATCAAT
    ACACCTGATTTAATCGCTGCAGTGAACGATGGTACTTTGTTAGGTGCTGCGATTGATACT
    TATGAAAATGAAGCAGCATACTTCACAAATGACTGGACTAATAAAGACATTGACGATAAA
    ACATTATTAGAGTTAATCGAACATGAAAGAATTTTAGTAACACCACATATTGCTTTCTTC
    TCTGATGAAGCAGTACAAAACCTTGTTGAAGGTGGTTTAAACGCAGCATTATCTGTCATT
    AACACTGGTACATGTGAAACACGTTTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS13280 [old locus tag: SACOL2535 ]
  • symbol: SACOL_RS13280
  • description: lactate dehydrogenase
  • length: 330
  • theoretical pI: 4.89771
  • theoretical MW: 36681.4
  • GRAVY: -0.193636

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.1.1.28?  ExPASy
    D-lactate dehydrogenase (R)-lactate + NAD+ = pyruvate + NADH
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 154.7)
    and 11 more
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 19.7)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 18.3)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 17.1)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 16)
    Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase (TIGR01723; EC 1.12.98.2; HMM-score: 14.7)
    Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 13.8)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 13.5)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 13)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 13)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 12.1)
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 11.6)
  • TheSEED: see SACOL2535
  • ⊞PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 166.1)
    and 12 more
    2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 86.6)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.2)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 25.1)
    KARI_N; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 21.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.4)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 15.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 15.6)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.5)
    HMD_N; HMD N-terminal domain (PF22616; HMM-score: 14.7)
    ThiF; ThiF family (PF00899; HMM-score: 12.2)
    Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.983
    • Cytoplasmic Membrane Score: 0.0009
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.016
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009748
    • TAT(Tat/SPI): 0.000599
    • LIPO(Sec/SPII): 0.001647
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLKDYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYSPETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAATAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDKAMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKDIDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2535
  • protein localization: see SACOL2535
  • quantitative data / protein copy number per cell: see SACOL2535
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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