PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS03450 [old locus tag: SACOL0667 ]
pan locus tag^?: SAUPAN002478000
symbol: SACOL_RS03450
pan gene symbol^?: —
• synonym:
• product: HAD family hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS03450 [old locus tag: SACOL0667 ]
• symbol: SACOL_RS03450
• product: HAD family hydrolase
• replicon: chromosome
• strand: +
• coordinates: 695745..696464
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (695745..696464) NCBI
• BioCyc: SACOL_RS03450 BioCyc
• MicrobesOnline: see SACOL0667

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGATTTGAATCAAATTAAAGCAGTTGTATTTGATTTAGAAGGTACGTTGTTGGACAGA
  GTTAAATCTCGAGAGAAATTTATCGAAGAGCAATATGAACGATTTCATGACTACTTAATT
  CATGTTCAACTGGCAGATTTTAAAAAAGCATTTATTGAGCTAGATGACGATGAAGATAAT
  GATAAACCTGATTTATATAAAGAAATCATTAAACGTTTCCATGTAGATAGGTTAACTTGG
  AAAGACTTATTTAATGATTTTGAAATGCATTTTTATCGTTATGTATTTCCTTATTACGAT
  ACTTTGTATACACTAGAAAAGCTATCGCAAAAAGGCTTTCAAATTGGTGTTATCGCAAAT
  GGTAAATCTAAGATTAAACAATTTCGATTACATTCACTTGGTTTGATGCATGTTATTAAT
  TATTTATCAACATCAGAAACAGTTGGTTTTCGTAAACCACATCCTAAAATTTTTGAAGAT
  ATGATTGATCAACTAGGGGTATTACCTGAGCAAATTATGTATGTTGGCGATGATGCGTTA
  AATGATGTAGCTCCAGCACGAGCTATGGGCATGGTTAGTGTATGGTATAAACAAGAAGAT
  GCTGAAATTGAACCACTCGAAGAAGAAGTTGATTTTACAATTACAACAGTGGAAGAATTA
  TTAACCATTTTACCAATAAAAAATGATAATAAAGGAGAAAATTATGGATCTATTTACTAG
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⊟Protein

⊟General

• locus tag: SACOL_RS03450 [old locus tag: SACOL0667 ]
• symbol: SACOL_RS03450
• description: HAD family hydrolase
• length: 239
• theoretical pI: 4.54953
• theoretical MW: 28196.9
• GRAVY: -0.401255

⊟Function

• ⊞TIGRFAM:
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 125.6)
  and 25 more
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 80.2)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 77.8)
  haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 70.5)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 61.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 52.4)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 50.8)
  AHBA synthesis associated protein (TIGR01454; HMM-score: 50.3)
  epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 48.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 40.3)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 39)
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 35)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 32.7)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 31.1)
  pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 27.1)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 26.4)
  HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 25.5)
  HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 23.3)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 22.6)
  Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 17.9)
  phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 17.1)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 16.7)
  HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 16)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 14.1)
  phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 12.3)
• TheSEED: see SACOL0667
• ⊞PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 91.1)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 79)
  and 4 more
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 38.3)
  PNK3P; Polynucleotide kinase 3 phosphatase (PF08645; HMM-score: 20.9)
  PGP_phosphatase; Mitochondrial PGP phosphatase (PF09419; HMM-score: 20.4)
  TPR (CL0020) Rgg_C; HTH-type transcriptional regulator Rgg, C-terminal domain (PF21259; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9768
  • Cytoplasmic Membrane Score: 0.018
  • Cell wall & surface Score: 0.0009
  • Extracellular Score: 0.0043
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000776
  • TAT(Tat/SPI): 0.000142
  • LIPO(Sec/SPII): 0.000144
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446287499 NCBI
• RefSeq: WP_000365354 NCBI
• UniProt: see SACOL0667

⊟Protein sequence

• MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQLADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTLYTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFEDMIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVEELLTILPIKNDNKGENYGSIY

⊟Experimental data

• experimentally validated: see SACOL0667
• protein localization: see SACOL0667
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications