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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1314 [new locus tag: SACOL_RS06700 ]
  • pan locus tag?: SAUPAN003598000
  • symbol: SACOL1314
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1314 [new locus tag: SACOL_RS06700 ]
  • symbol: SACOL1314
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1329661..1330152
  • length: 492
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAATCAAGAAAACTGGCTATAACTGCACTTTTAATTGCAATAAATGTTGTATTAAGC
    AGTATTATCATCATTCCTCTAGGACCAGTTAAGGCAGCACCAGTACAGCATTTTGTAAAT
    GTATTAAGTGCGGTCATAGTAGGTCCTTGGTATGGATTAGCTCAAGCGCTTATATCATCA
    ATTTTAAGAGTTCTTTTTGGTACTGGTACAGCTTTTGCATTTCCGGGTAGTATGATTGGA
    GTTTTATTGGCTAGTATGTTTTACATATATCGTAAACATATATTTATGGCCGCGGTCGGT
    GAAGTACTTGGAACTGGTGTCATCGGAAGTTTAATTTGTATACCATTAGCATATTTCCTT
    GGGCTTCAAGACTTCTTCATTAAACCGTTAATGATTACGTTCATAGTCTCAAGTGCTATC
    GGATCTATTATAAGTTATTTCTTATTAATTACTCTAAAAAAACGTGGTATTCTTCAAAGG
    TTTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1314 [new locus tag: SACOL_RS06700 ]
  • symbol: SACOL1314
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 10.9181
  • theoretical MW: 17600.5
  • GRAVY: 1.23067

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
    Metabolism Transport and binding proteins Other energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
    and 2 more
    Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 36.3)
    Cell structure Cell envelope Surface structures PEP-CTERM protein-sorting domain (TIGR02595; HMM-score: 9)
  • ⊞TheSEED  :
    • Substrate-specific component ThiW of predicted thiazole ECF transporter
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Substrate-specific component ThiW of predicted thiazole ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component ThiW of predicted thiazole ECF transporter
  • ⊞PFAM:
    Gx_transp (CL0315) ThiW; Thiamine-precursor transporter protein (ThiW) (PF09512; HMM-score: 185.9)
    and 2 more
    ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 46.1)
    ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 25.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9991
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0007
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.879749
    • TAT(Tat/SPI): 0.014507
    • LIPO(Sec/SPII): 0.003793
    • Cleavage Site: CS pos: 32-33. VKA-AP. Pr: 0.8741
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSAVIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEVLGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQRFIK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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