PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2100 [new locus tag: NWMN_RS12150 ]
pan locus tag^?: SAUPAN005594000
symbol: lacR
pan gene symbol^?: lacR
• synonym:
• product: lactose phosphotransferase system repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2100 [new locus tag: NWMN_RS12150 ]
• symbol: lacR
• product: lactose phosphotransferase system repressor
• replicon: chromosome
• strand: -
• coordinates: 2337623..2338378
• length: 756
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332066 NCBI
• RefSeq: YP_001333134 NCBI
• BioCyc:
• MicrobesOnline: 3707702 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAATAAACATGAACGTTTGGACGAAATTGCTAAACTAGTGAATAAAAAGGGCACGATA
  AGAACGAATGAAATCGTCGAAGGTTTAAATGTGTCTGATATGACAGTTCGAAGAGATTTA
  ATTGAATTGGAAAATAAAGGGATTTTAACGAAGATTCATGGTGGTGCACGCAGTAATTCA
  ACATTTCAGTATAAAGAAATATCGCATAAAGAAAAACATACCCGTCAAATTGCAGAAAAA
  CGATTTATAGCTAAAAAAGCTGCATCATTAATTGAAGATGGGGATACTTTGTTTTTCGGA
  CCAGGAACAACAGTGGAACTATTAGCAGAAGAAGTCAATCATCATACGCTCACAATTATT
  ACGAATTGTTTGCCGGTGTATAAAATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTC
  TATTTAATTGGTGGTGAAATGCGCCATATTACAGAAGCATTTGTAGGTGAAATGGCGAAC
  GCCATGTTGGAAAAACTAAGATTTAGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAA
  GGTGCTGTGATGACATCTACATTAGATGAAGCCTATACGCAACAACTGGCACTAAGTAAT
  TCAATTGAAAAATACTTGTTAATCGATCATACGAAAGTTGGCAAAGAAGATTTTACATCA
  TTTTGTCAGTTAAACGAATTGACTGCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTA
  GAAACGATTAAAACATATATTGAAGTAGTCGACTAA
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⊟Protein

⊟General

• locus tag: NWMN_2100 [new locus tag: NWMN_RS12150 ]
• symbol: LacR
• description: lactose phosphotransferase system repressor
• length: 251
• theoretical pI: 6.24703
• theoretical MW: 28548.6
• GRAVY: -0.293626

⊟Function

• ⊞TIGRFAM:
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 23.8)
  and 4 more
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 15.8)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 15.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
  Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
• TheSEED  :
  • Lactose phosphotransferase system repressor
  Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Lactose phosphotransferase system repressor
• ⊞PFAM:
  NADP_Rossmann (CL0063) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 164.8)
  and 11 more
  HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 81.1)
  HTH_11; HTH domain (PF08279; HMM-score: 26.4)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.3)
  FaeA; FaeA-like protein (PF04703; HMM-score: 17.7)
  DUF7343; Domain of unknown function (DUF7343) (PF24034; HMM-score: 15.7)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 15.6)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14.8)
  NADP_Rossmann (CL0063) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 14)
  HTH (CL0123) Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 13.9)
  PaaX; PaaX-like protein (PF07848; HMM-score: 12.4)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by LacR, TF important in Lactose utilization: in N315

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9975
  • Cytoplasmic Membrane Score: 0.0007
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0017
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024817
  • TAT(Tat/SPI): 0.00181
  • LIPO(Sec/SPII): 0.000503
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222312 NCBI
• RefSeq: YP_001333134 NCBI
• UniProt: A0A0H3K9X1 UniProt

⊟Protein sequence

• MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications