⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_01505
pan locus tag^?: SAUPAN003950000
symbol: SAOUHSC_01505
pan gene symbol^?: ribU
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_01505
• symbol: SAOUHSC_01505
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1458085..1458630
• length: 546
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3919048 NCBI
• RefSeq: YP_500023 NCBI
• BioCyc: G1I0R-1398 BioCyc
• MicrobesOnline: 1289937 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGCAACAAAATAAACGTCTTATCACAATAAGTATGTTGAGCGCGATTGCGTTTGTGTTA
  ACTTTTATCAAGTTTCCTATACCATTTTTGCCACCATACTTAACTTTAGATTTTAGTGAT
  GTACCGTCACTACTAGCTACATTTACGTTTGGACCAGTTGCCGGTATCGTAGTTGCACTG
  GTTAAAAATTTATTGAACTACTTATTTAGTATGGGCGATCCAGTTGGACCATTTGCTAAC
  TTTTTAGCAGGCGCAAGTTTCTTATTAACTGCTTACGCCATCTATAAAAATAAACGTTCA
  ACAAAATCTTTGATTACTGGATTAATCATTGCAACAATCGTTATGACTATCGTGTTGAGT
  ATTTTGAACTATTTCGTTCTATTACCTTTGTACGGTATGATATTTAACTTAGCTGATATC
  GCAAATAATCTTAAAGTAATCATTGTTTCAGGAATTATACCATTCAATATTATTAAAGGT
  ATCGTTATTTCTATTGTATTTATTTTACTATATAGAAGGCTTGCGAATTTCTTGAAAAGA
  ATTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  546
  

⊟Protein

⊟General

• locus tag: SAOUHSC_01505
• symbol: SAOUHSC_01505
• description: hypothetical protein
• length: 181
• theoretical pI: 10.6184
• theoretical MW: 20024.3
• GRAVY: 1.10552

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 34.4)
  and 1 more
  membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (TIGR03074; EC 1.1.-.-; HMM-score: 9.2)
• ⊞⊞TheSEED  :
  • Riboflavin ECF transporter, substrate-specific component RibU
  Cofactors, Vitamins, Prosthetic Groups, Pigments Riboflavin, FMN, FAD Riboflavin, FMN and FAD metabolism Extended  Substrate-specific component RibU of riboflavin ECF transporter
  and 1 more
  Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component RibU of riboflavin ECF transporter
• ⊞⊞PFAM:
  Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 132.9)
  and 9 more
  ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 45.4)
  Thia_YuaJ; Thiamine transporter protein (Thia_YuaJ) (PF09515; HMM-score: 21.9)
  5TM-5TMR_LYT; 5TMR of 5TMR-LYT (PF07694; HMM-score: 20.9)
  CbiM; Cobalt uptake substrate-specific transmembrane region (PF01891; HMM-score: 18.1)
  Hpre_diP_synt_I; Heptaprenyl diphosphate synthase component I (PF07456; HMM-score: 16)
  no clan defined DUF4118; Domain of unknown function (DUF4118) (PF13493; HMM-score: 16)
  EBP; EXPERA (EXPanded EBP superfamily) (PF05241; HMM-score: 14.2)
  Tmemb_14; Transmembrane proteins 14C (PF03647; HMM-score: 13.7)
  Peroxisome (CL0484) Pex24p; Integral peroxisomal membrane peroxin (PF06398; HMM-score: 6.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0008
• ⊞⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.05587
  • TAT(Tat/SPI): 0.000847
  • LIPO(Sec/SPII): 0.010773
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 88195221 NCBI
• RefSeq: YP_500023 NCBI
• UniProt: Q2FYE7 UniProt
• STRING: 93061.SAOUHSC_01505 STRING

⊟Protein sequence

• MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLKRI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• predicted SigA promoter  : SAOUHSC_01505 < S626

⊟Regulation

• ⊞⊞regulator: FMN-box (transcription termination) regulon

  FMN-box

  (5' cis-acting region)

  important in Riboflavin biosynthesis; compare RegPrecise for N315

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications