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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0933 [new locus tag: NWMN_RS05225 ]
  • pan locus tag?: SAUPAN003277000
  • symbol: purE
  • pan gene symbol?: purE
  • synonym:
  • product: phosphoribosylaminoimidazole carboxylase catalytic subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0933 [new locus tag: NWMN_RS05225 ]
  • symbol: purE
  • product: phosphoribosylaminoimidazole carboxylase catalytic subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1036095..1036577
  • length: 483
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    GTGAAAGTAGCAGTCATTATGGGCAGTTCTTCCGATTGGAAAATTATGCAAGAGAGTTGT
    AACATGTTGGATTATTTTGAAATTCCGTACGAAAAACAAGTAGTATCCGCACATCGTACG
    CCAAAAATGATGGTTCAATTTGCTTCTGAAGCGAGAGAAAGAGGTATAAACATTATCATT
    GCAGGCGCTGGCGGTGCGGCACATTTACCAGGTATGGTTGCATCATTAACGACGCTACCA
    GTTATTGGAGTGCCGATTGAAACAAAAAGTTTAAAGGGTATAGATTCTTTATTATCAATT
    GTTCAAATGCCAGGAGGTATTCCGGTTGCAACGACTGCAATTGGTGCAGCAGGTGCTAAA
    AACGCAGGTATACTTGCAGCAAGAATGTTAAGTATTCAAAATCCTTCTTTAGTTGAAAAA
    CTAAATCAGTATGAATCTTCGTTAATTCAAAAAGTGGAGGACATGCAAAATGAACTTCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    483

⊟Protein[edit | edit source]

Protein Data Bank: 3ORS

⊟General[edit | edit source]

  • locus tag: NWMN_0933 [new locus tag: NWMN_RS05225 ]
  • symbol: PurE
  • description: phosphoribosylaminoimidazole carboxylase catalytic subunit
  • length: 160
  • theoretical pI: 6.52439
  • theoretical MW: 17068.9
  • GRAVY: 0.189375

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.4.99.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide mutase 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, catalytic subunit (TIGR01162; EC 4.1.1.21; HMM-score: 217.1)
  • TheSEED  :
    • N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21)
  • PFAM:
    DHQS (CL0224) AIRC; AIR carboxylase (PF00731; HMM-score: 215.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9141
    • Cytoplasmic Membrane Score: 0.0056
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0798
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018469
    • TAT(Tat/SPI): 0.000661
    • LIPO(Sec/SPII): 0.007281
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMMVQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQMPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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