PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS04400 [old locus tag: SA0770 ]
pan locus tag^?: SAUPAN002839000
symbol: SA_RS04400
pan gene symbol^?: metP1
• synonym:
• product: ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS04400 [old locus tag: SA0770 ]
• symbol: SA_RS04400
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 876616..877311
• length: 696
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (876616..877311) NCBI
• BioCyc: SA_RS04400 BioCyc
• MicrobesOnline: see SA0770

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGTAAATCATTTAGTGAAATTATAAATGAAATGATTACAATGCCTAATATTCAGTGG
  CCAGAAGTTTGGACTGCAATAGTCGAAACACTATACATGACAGTCGTCTCAACTATATTT
  GCATTTATACTCGGTCTTATTTTAGGTGTGTTATTATTCTTGTCTGCTAAAGGTAAGTCT
  ATCGGTGCAAGGTTATTTTATTCTATCGTTTCTTTCATTGTTAACTTATTTAGAGCGATA
  CCATTTATTATTTTAATTTTATTATTAATTCCATTTACAAGTTTGATACTTGGAACGATA
  AGTGGTCCGACAGGTGCGTTACCAGCCTTGATTATTGGCGCAGCACCGTTTTATGCAAGG
  CTCGTAGAAATTGCTTTTAAAGAAATTGATAAAGGTGTCATCGAAGCGGCCTGGTCAATG
  GGTGCCAATACTTGGATAGTAATTCGTAAAGTCCTCTTACCTGAAGCCATGCCAGCGCTA
  GTGTCTGGCATTACAGTTACAGCAATCGCTTTAGTTGGTTCAACAGCAGTTGCAGGTGTA
  ATTGGTGCCGGTGGTTTAGGAAATTTAGCATACTTAACAGGTTTCACTCGAAATCAAAAT
  GATGTCATTTTAGTATCAACTGTTTTTATTTTAATTATTGTATTTATAATCCAATTCATT
  GGGGATTGGCTTACAAATAAACTTGATAAACGATAA
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  696
  

⊟Protein

⊟General

• locus tag: SA_RS04400 [old locus tag: SA0770 ]
• symbol: SA_RS04400
• description: ABC transporter permease
• length: 231
• theoretical pI: 9.46842
• theoretical MW: 24902.8
• GRAVY: 1.09221

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 84.6)
  and 15 more
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 51.9)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 46.4)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 37)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 34.8)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 34.3)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 32.4)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 26.4)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 26.3)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 24.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 23.9)
  ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 22.6)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 12.6)
  Metabolism Energy metabolism Electron transport cytochrome aa3 quinol oxidase, subunit II (TIGR01432; EC 1.10.3.-; HMM-score: 9.4)
  Metabolism Energy metabolism Electron transport caa(3)-type oxidase, subunit IV (TIGR02229; HMM-score: 7)
• TheSEED: see SA0770
• ⊞PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.8)
  and 2 more
  no clan defined TEX13B_C; Testis-expressed protein 13B, C-terminal (PF22836; HMM-score: 11)
  DUF6773; Family of unknown function (DUF6773) (PF20563; HMM-score: 7.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009004
  • TAT(Tat/SPI): 0.000133
  • LIPO(Sec/SPII): 0.001426
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446442376 NCBI
• RefSeq: WP_000520231 NCBI
• UniProt: see SA0770

⊟Protein sequence

• MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFILGLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISGPTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWIVIRKVLLPEAMPALVSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQFIGDWLTNKLDKR

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: S-box, CodY see SA0770

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications