⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1421 [new locus tag: SA_RS08020 ]
pan locus tag^?: SAUPAN004179000
symbol: SA1421
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1421 [new locus tag: SA_RS08020 ]
• symbol: SA1421
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1626895..1627479
• length: 585
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124263 NCBI
• RefSeq: NP_374706 NCBI
• BioCyc: see SA_RS08020
• MicrobesOnline: 103732 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGAACATTGAAAAAGCAAAACGGCTTGCAAAAGAGAAACTGCCAGAGAAACGTTATAAT
  CATTCTTTAAGAGTTGCAGAAACAGCGATTAAATTGGCAGAAATTTATGATGGAGACACT
  AGTAAAGTAGAATTAGCAGGTGTATTACATGATTTCTGTAAATATGATGATTTAGGTAAA
  ATGTATCAAATTGTTCGACAATACGAATTAGGTAATGATCTACTGAGTTATGGTAGTGAA
  ATATTGCATGGCCCTGTGTGTGCAGCAATCATGGAACATGAATATGGTATCAATGATGAG
  GAAGTATTAATGGCTATCAAATACCATACTACTGGACGTCAACAAATGACAAAAACTGAA
  AAACTGATTTTTATTGCTGATTACATCGAACCTGGAAGAACAATCCCAGGAGTTGATGAT
  ATTCGAGATATGGCATACAATCAAGGTAGTTTAGATAAGACAATTTATGAAATTTCTAAA
  CGCACAGTACTATTTTTAATACAGAAAGATATTACGGTATATAATAAGACGATTGACTGT
  TTAAATTATTATAACTATAGTGACGAAAGAATAAAGGATGATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  585
  

⊟Protein

⊟General

• locus tag: SA1421 [new locus tag: SA_RS08020 ]
• symbol: SA1421
• description: hypothetical protein
• length: 194
• theoretical pI: 5.15494
• theoretical MW: 22463.5
• GRAVY: -0.49433

⊟Function

• ⊞⊞TIGRFAM:
  Unknown function Enzymes of unknown specificity putative HD superfamily hydrolase (TIGR00488; HMM-score: 140.2)
  and 4 more
  HDIG domain (TIGR00277; HMM-score: 24.5)
  Hypothetical proteins Conserved TIGR00295 family protein (TIGR00295; HMM-score: 18.9)
  HD domain protein, cyanamide hydratase family (TIGR03401; EC 4.2.1.-; HMM-score: 16.3)
  Genetic information processing Transcription Degradation of RNA ribonuclease Y (TIGR03319; EC 3.1.-.-; HMM-score: 11.2)
• TheSEED  :
  • Hydrolase (HD superfamily), YqeK
  IojapClusters  Hydrolase (HAD superfamily), YqeK
• ⊞⊞PFAM:
  HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 52.4)
  and 1 more
  no clan defined DUF5572; Family of unknown function (DUF5572) (PF17733; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9785
  • Cytoplasmic Membrane Score: 0.0034
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0179
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005836
  • TAT(Tat/SPI): 0.000227
  • LIPO(Sec/SPII): 0.000722
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927173 NCBI
• RefSeq: NP_374706 NCBI
• UniProt: A0A0H3JMU3 UniProt

⊟Protein sequence

• MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVELAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEVLMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISKRTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1419 < SA1420 < SA1421 < SA1422 < SA1423 < aroE < SA1425 < SA1426 < pfs

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications