From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS12245 [old locus tag: SACOL2329 ]
  • pan locus tag?: SAUPAN005825000
  • symbol: SACOL_RS12245
  • pan gene symbol?: rpiA
  • synonym:
  • product: ribose-5-phosphate isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS12245 [old locus tag: SACOL2329 ]
  • symbol: SACOL_RS12245
  • product: ribose-5-phosphate isomerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2392091..2392777
  • length: 687
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2392091..2392777) NCBI
  • BioCyc: SACOL_RS12245 BioCyc
  • MicrobesOnline: see SACOL2329

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGATGTCAAAGCACTCAAGTTAATGACATTAAATGATGTTTTAAGTCAAATCAAT
    GGTGACATGACGCTGGGAATTGGTACAGGAAGTACAATGGAATTACTCTTACCTCAAATG
    GCGCAACTAATTAAAGAACGTGGTTACAATATTACAGGTGTATGCACTTCTAATAAAATT
    GCATTTTTAGCAAAAGAATTAGGTATTAAAATATGTGAAATCAATGATGTTGATCATATC
    GACTTAGCAATTGATGGTGCTGATGAAGTAGATCCATCTTTAAATATAATTAAAGGTGGC
    GGTGGTGCGCTGTTCAGAGAGAAAGTTATAGATGAAATGGCATCGCGTTTTGTTGTGGTT
    GTCGATGAAACGAAAATAGTTCAATACTTAGGTGAGACGTTTAAGTTACCAGTAGAGGTG
    GATAAATTTAACTGGTATCAGATATTACGTAAAATTGAATCATACGCTGATATAAAAGTA
    GAACGTCGTGTAAACGAAGATGTCGCATTTATAACTGATAATGGCAATTATATTTTAGAT
    TGTAAGTTACCAAAAGGAATTGACCCATATAAGTTCCATGAGTATTTAATTCATCTGACA
    GGTGTGTTTGAGACAGGATACTTCTTAGATATGGCAGATCAAGTTATTGTAGGCACTCAA
    GAGGGTGTTAAAATTTTAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    687

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS12245 [old locus tag: SACOL2329 ]
  • symbol: SACOL_RS12245
  • description: ribose-5-phosphate isomerase
  • length: 228
  • theoretical pI: 4.54963
  • theoretical MW: 25540.4
  • GRAVY: 0.0192982

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.3.1.6?  ExPASy
    Ribose-5-phosphate isomerase D-ribose 5-phosphate = D-ribulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase A (TIGR00021; EC 5.3.1.6; HMM-score: 262.1)
  • TheSEED: see SACOL2329
  • ⊞PFAM:
    NADP_Rossmann (CL0063) Rib_5-P_isom_A; Ribose 5-phosphate isomerase A (phosphoriboisomerase A) (PF06026; HMM-score: 200.9)
    and 1 more
    DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9974
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0024
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012933
    • TAT(Tat/SPI): 0.001009
    • LIPO(Sec/SPII): 0.001056
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLIKERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGGALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYQILRKIESYADIKVERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVGTQEGVKILEK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL2329
  • protein localization: see SACOL2329
  • quantitative data / protein copy number per cell: see SACOL2329
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS12245&oldid=101805"