⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS14310 [old locus tag: SACOL2738 ]
pan locus tag^?: SAUPAN006489000
symbol: SACOL_RS14310
pan gene symbol^?: trmE
• synonym:
• product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS14310 [old locus tag: SACOL2738 ]
• symbol: SACOL_RS14310
• product: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE
• replicon: chromosome
• strand: -
• coordinates: 2807106..2808485
• length: 1380
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2807106..2808485) NCBI
• BioCyc: SACOL_RS14310 BioCyc
• MicrobesOnline: see SACOL2738

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGATTTAGATACAATTACGAGTATTTCAACACCAATGGGTGAAGGGGCAATTGGAATT
  GTTCGATTGTCTGGACCGCAAGCCGTTGAAATTGCTGACAAATTATATAAAGGAAAACAT
  CTTTTAAATGATGTTCCATCACATACGATTAATTACGGTCATATTATTGATCCAGAGTCT
  AAAGAAGTGATTGAAGAAGTTATGGTGTCTGTGTTAAGAGCGCCAAAAACATTTACACGC
  GAAGATATTATAGAAATTAATTGTCATGGTGGTATTTTAACGATTAATAGAGTGCTGGAA
  CTTACTATGACATATGGCGCTAGAATGGCTGAGCCAGGTGAATTTACAAAACGTGCCTTT
  TTAAATGGTCGTATTGATTTATCTCAAGCTGAAGCAGTTATGGACTTTATTCGCTCGAAG
  ACAGATAGAGCTTCTAAAGTTGCGATGAATCAAATTGAAGGTCGTCTAAGTGACTTAATC
  AAAAAACAACGTCAATCTATATTAGAGATACTCGCTCAAGTGGAAGTGAATATTGATTAT
  CCTGAATACGATGATGTTGAAGATGCGACTACTGAATTTCTTTTAGAGCAGTCTAAAGAA
  ATCAAACAGGAAATTAATCGTTTATTAGATACCGGTGCGCAGGGTAAAATTATGCGTGAA
  GGTTTATCTACAGTTATTGTTGGTAAACCAAACGTAGGTAAATCATCGATGTTAAATAAT
  TTAATACAAGATAATAAAGCGATTGTAACTGAGGTAGCAGGTACTACTAGAGATGTCTTA
  GAAGAGTACGTCAATGTTCGTGGCGTGCCATTAAGATTAGTTGATACTGCTGGTATACGT
  GAGACAGAAGATATAGTAGAGAAGATTGGTGTTGAACGCTCTAGAAAGGCTCTTAGCCAA
  GCAGACTTAATTTTATTTGTATTAAACAATAACGAAGCATTGACTCAAGAAGATTACACA
  TTATATGAAGTGGTTAAAAATGAAGATGTAATCGTAATTGTTAATAAAATGGATTTAGAG
  CAAAACATAGATATTAATGAAGTTAAAGATATGATAGGTGATACGCCATTAATTCAAACT
  TCAATGTTAAAACAAGAAGGTATTGATGAATTAGAAATACAAATTCGAGATTTGTTCTTT
  GGTGGAGAAGTACAAAATCAAGATATGACTTATGTTTCTAATTCAAGACATATTTCATTA
  TTAAAACAAGCAAGACAAACGATACAAGATGCGATTGATGCAGCAGAATCTGGTGTGCCT
  ATGGATATGGTACAAATTGATTTAACTAGAACTTGGGAAATATTAGGAGAAATTATTGGT
  GAGACTGCAAGTGATGAACTCATCGATCAGTTATTCAGTCAATTCTGCTTAGGTAAATAG
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⊟Protein

⊟General

• locus tag: SACOL_RS14310 [old locus tag: SACOL2738 ]
• symbol: SACOL_RS14310
• description: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE
• length: 459
• theoretical pI: 4.30301
• theoretical MW: 51355.2
• GRAVY: -0.206536

⊟Function

• reaction:
  EC 3.6.-.-?  ExPASy
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 434.5)
  and 13 more
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 115.9)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 91.5)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 90.6)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 67.9)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 53.1)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 48.9)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 38)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 25.9)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 22.7)
  Metabolism Transport and binding proteins Amino acids, peptides and amines chloroplast protein import component Toc86/159, G and M domains (TIGR00993; HMM-score: 20.7)
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 19.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 14.3)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines GTP-binding protein (TIGR00991; HMM-score: 14.1)
• TheSEED: see SACOL2738
• ⊞⊞PFAM:
  no clan defined MnmE_helical; MnmE helical domain (PF12631; HMM-score: 211)
  and 12 more
  FBD (CL0289) TrmE_N; GTP-binding protein TrmE N-terminus (PF10396; HMM-score: 146.2)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 94.9)
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 44.6)
  Ras; Ras family (PF00071; HMM-score: 29.5)
  Dynamin_N; Dynamin family (PF00350; HMM-score: 29.2)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 28.2)
  AIG1; AIG1 family (PF04548; HMM-score: 28)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 24.1)
  Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 21.9)
  Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 20.8)
  GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 12.3)
  P-loop_NTPase (CL0023) Arf; ADP-ribosylation factor family (PF00025; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: K⁺
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9682
  • Cytoplasmic Membrane Score: 0.0311
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0006
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009151
  • TAT(Tat/SPI): 0.000938
  • LIPO(Sec/SPII): 0.001923
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446284648 NCBI
• RefSeq: WP_000362503 NCBI
• UniProt: see SACOL2738

⊟Protein sequence

• MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLNDVPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELTMTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLIKKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIMREGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDTAGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIVNKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYVSNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQLFSQFCLGK

⊟Experimental data

• experimentally validated: see SACOL2738
• protein localization: see SACOL2738
• quantitative data / protein copy number per cell: see SACOL2738
• ⊟⊟interaction partners:

  SACOL_RS00185		PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA   (data from MRSA252)
  SACOL_RS06505		30S ribosomal protein S2   (data from MRSA252)
  SACOL_RS11435		Asp23/Gls24 family envelope stress response protein   (data from MRSA252)
  SACOL_RS11695		30S ribosomal protein S5   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications