⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS13810 [old locus tag: SACOL2638 ]
pan locus tag^?: SAUPAN006325000
symbol: SACOL_RS13810
pan gene symbol^?: cysG
• synonym:
• product: precorrin-2 dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS13810 [old locus tag: SACOL2638 ]
• symbol: SACOL_RS13810
• product: precorrin-2 dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2698041..2698646
• length: 606
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2698041..2698646) NCBI
• BioCyc: SACOL_RS13810 BioCyc
• MicrobesOnline: see SACOL2638

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAATATGCCATTAATGATAGATTTAACAAATAAAAATGTCGTCATAGTTGGTGGAGGC
  GTCGTTGCAAGTCGTCGGGCACAAACATTAAATCAATACGTTGAACATATGACGGTCATC
  AGTCCGACAATCACTGAAAAACTTCAAAATATGGTAGATAACGGTGTCGTCATATGGAAA
  GAAAAAGAATTTGAACCAAGCGATATTGTAGACGCGTATCTAGTTATTGCAGCAACCAAT
  GAGCCACGTGTCAATGAAGCGGTAAAAAAAGCCTTACCTGAGCATGCCCTTTTTAATAAT
  GTTGGAGATGCATCAAATGGCAATGTTGTATTTCCAAGTGCACTACACCGCGACAAGCTA
  ACTATCAGTGTATCAACTGATGGTGCGAGTCCTAAGTTGACAAAATCAATTATGGCAGAG
  CTTGAGGCGTTATATCCACCATCATACAGTTCGTATATCGACTTTTTATATACTTGCCGA
  CAGAAAATAAAAGTACTTGATATAACATATAACGAAAAGCAACAGTTACTGTCACAAATT
  GTGTCACAAGAATATTTAAATCATGACAAACAAGCTCAATTTTTAGCGTGGTTGGATGTA
  AGATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  606
  

⊟Protein

⊟General

• locus tag: SACOL_RS13810 [old locus tag: SACOL2638 ]
• symbol: SACOL_RS13810
• description: precorrin-2 dehydrogenase
• length: 201
• theoretical pI: 5.66459
• theoretical MW: 22541.7
• GRAVY: -0.146766

⊟Function

• ⊞⊞reaction:
  EC 1.3.1.76  ExPASy
  Precorrin-2 dehydrogenase Precorrin-2 + NAD⁺ = sirohydrochlorin + NADH?
• ⊞⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin siroheme synthase, N-terminal domain (TIGR01470; HMM-score: 113.1)
  and 1 more
  Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 14.4)
• TheSEED: see SACOL2638
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 91.3)
  and 7 more
  no clan defined SirC_C; SirC C-terminal domain (PF22440; HMM-score: 61.6)
  Sirohm_synth_M; Sirohaem biosynthesis protein central (PF14824; HMM-score: 27.1)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 20.4)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 17.9)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 16.4)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.4)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9808
  • Cytoplasmic Membrane Score: 0.019
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0001
• LocateP:
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015806
  • TAT(Tat/SPI): 0.001753
  • LIPO(Sec/SPII): 0.005276
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446979795 NCBI
• RefSeq: WP_001057051 NCBI
• UniProt: see SACOL2638

⊟Protein sequence

• MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTITEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVGDASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCRQKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR

⊟Experimental data

• experimentally validated: see SACOL2638
• protein localization: see SACOL2638
• quantitative data / protein copy number per cell: see SACOL2638
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications