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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1423 [new locus tag: SACOL_RS07245 ]
  • pan locus tag?: SAUPAN003796000
  • symbol: SACOL1423
  • pan gene symbol?: pstC
  • synonym:
  • product: phosphate ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1423 [new locus tag: SACOL_RS07245 ]
  • symbol: SACOL1423
  • product: phosphate ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1434019..1434945
  • length: 927
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3236372 NCBI
  • RefSeq: YP_186275 NCBI
  • BioCyc: see SACOL_RS07245
  • MicrobesOnline: 912881 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTTCATCTACTAATGTTAAAGCTTTAATCGAAAAAAATAATAATAAAAAAGGAAAG
    CATAATGACAAAATTATACCAGTTATTTTAGCCGCAATTTCAGCGATTTCCATTTTAACA
    ACACTAGGTATATTAATCACATTGCTTTTAGAAACCATCACTTTTTTCACCAGAATTCCA
    ATAACTGAATTTCTATTTTCTACTACTTGGAATCCTACCGGTTCAGACCCTAAGTTTGGT
    ATCTGGGCATTGATAATAGGGACTTTAAAAATCACAGTTATTGCGACTATATTTGCAGTT
    CCAGTCGGGCTTGGTGCAGCAATTTATTTAAGTGAATATGCAAGTGACCGTGCACGACGC
    ATAATTAAACCGATATTAGAAATTTTAGCAGGAATACCAACAATTGTGTTTGGTTTCTTT
    GCATTAACCTTTGTTACACCAGTATTAAGATCTTTCATACCAGGTCTTGGAGAGTTTAAT
    GCTATAAGTCCCGGCTTAGTTGTCGGTATTATGATTGTCCCTCTCATCACAAGTTTGAGT
    GAGGATGCAATGGCATCTGTACCAAATAAAATTCGAGAAGGTGCCTATGGACTTGGAGCA
    ACTAAATTAGAAGTAGCAACTAAAGTCGTACTTCCCGCAGCAACATCAGGTATTGTAGCT
    TCAATCGTTCTCGCGATTTCAAGAGCAATTGGAGAAACGATGATTGTATCATTAGCGGCA
    GGTAGTTCGCCAACAGCTTCATTAAGTTTAACAAGTTCGATTCAAACAATGACTGGATAT
    ATTGTTGAGATAGCGACAGGTGATGCAACATTTGGATCAAATATTTATTACAGTATTTAT
    GCTGTAGGGTTCACACTATTTATCTTTACCTTAATCATGAATTTACTTTCTCAGTGGATT
    TCTAAGCGTTTTAGGGAGGAGTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1423 [new locus tag: SACOL_RS07245 ]
  • symbol: SACOL1423
  • description: phosphate ABC transporter permease
  • length: 308
  • theoretical pI: 9.67025
  • theoretical MW: 32903.6
  • GRAVY: 0.744156

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 265.7)
    and 9 more
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 177.6)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 53.5)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 49.4)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 35)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 32.6)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 30.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 20.8)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 20.5)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 16.3)
  • ⊞TheSEED  :
    • Phosphate ABC transporter, permease protein PstC
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate transport system permease protein PstC (TC 3.A.1.7.1)
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate transport system permease protein PstC (TC 3.A.1.7.1)
  • ⊞PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 72)
    and 1 more
    no clan defined DUF4605; Domain of unknown function (DUF4605) (PF15378; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001337
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.001187
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

  • GI: 57651888 NCBI
  • RefSeq: YP_186275 NCBI
  • UniProt: A0A0H2WYU9 UniProt

⊟Protein sequence[edit | edit source]

  • MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGILITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPVGLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFNAISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGIVASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIYYSIYAVGFTLFIFTLIMNLLSQWISKRFREEY

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SACOL1420 < SACOL1421 < SACOL1422 < SACOL1423

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1423&oldid=94204"
  • This page was last edited on 11 March 2016, at 09:52.
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