PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA2234 [new locus tag: SA_RS12830 ]
pan locus tag^?: SAUPAN005997000
symbol: opuCD
pan gene symbol^?: opuCD
• synonym:
• product: glycine betaine/carnitine/choline ABC transporter opuCD

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2234 [new locus tag: SA_RS12830 ]
• symbol: opuCD
• product: glycine betaine/carnitine/choline ABC transporter opuCD
• replicon: chromosome
• strand: -
• coordinates: 2509481..2510176
• length: 696
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125161 NCBI
• RefSeq: NP_375557 NCBI
• BioCyc: see SA_RS12830
• MicrobesOnline: 104583 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGAAGGTAATTTATTACAGCAATTATTCAATTATTATGTTACGAACTTTGGTTATCTA
  TGGGATTTATTTTTCAAACACTTATTAATGTCTGTCTATGGTGTGCTGTTTGCAGCTTTA
  ATTGGTATTCCATTGGGAATCTTGCTTGCAAGATACACAAAACTTTCTGGATTTGTAATT
  ACAATTGCAAATATAATTCAAACAGTTCCAGTCATTGCAATGTTAGCTATTTTAATGTTA
  GTCATGGGCTTAGGTTCAGAAACAGTAGTTTTAACAGTGTTTTTATATGCGTTACTTCCA
  ATTATAAAAAACACTTATACTGGTATAGCTAGTGTTGATGCGAATATTAAGGATGCTGGC
  AAAGGTATGGGAATGACACGCAATCAAGTGCTACGAATGATTGAATTACCGTTATCTGTT
  TCGGTTATTATCGGTGGCATTCGTATTGCCTTGGTTGTTGCGATAGGTGTTGTTGCCGTT
  GGATCATTTATAGGAGCACCTACGCTTGGTGACATTGTGATTCGTGGTACAAATGCGACG
  GATGGCACAACGTTTATTTTAGCAGGTGCGATTCCGATTGCTATCATTGCAATCGTCATT
  GATGTACTATTAAGATTTTTAGAAAAACGATTAGACCCAACAACACGACATCGTAAAAAT
  CAATCTAATCATCGGCCGCAAAGTATTAATATGTAA
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⊟Protein

⊟General

• locus tag: SA2234 [new locus tag: SA_RS12830 ]
• symbol: OpuCD
• description: glycine betaine/carnitine/choline ABC transporter opuCD
• length: 231
• theoretical pI: 10.1502
• theoretical MW: 25025.9
• GRAVY: 0.844156

⊟Function

• ⊞TIGRFAM:
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 86.1)
  and 10 more
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 44.7)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 32.3)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 31.7)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 22)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 19.6)
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 18.4)
  Cellular processes Cellular processes Pathogenesis autotransporter-associated beta strand repeat (TIGR02601; HMM-score: 10)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking autotransporter-associated beta strand repeat (TIGR02601; HMM-score: 10)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 9.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides celllulose biosynthesis operon protein BcsF/YhjT (TIGR03493; HMM-score: 5.4)
• TheSEED  :
  • Choline ABC transport system, permease protein OpuBD
  Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Osmotically activated L-carnitine/choline ABC transporter, permease protein OpuCD
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 87.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001453
  • TAT(Tat/SPI): 0.000164
  • LIPO(Sec/SPII): 0.001504
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 15928024 NCBI
• RefSeq: NP_375557 NCBI
• UniProt: A0A0H3JRP7 UniProt

⊟Protein sequence

• MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIPLGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPIIKNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAVGSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHRKNQSNHRPQSINM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: opuCD < opuCC < opuCB < opuCA

⊟Regulation

• ⊞regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications