⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1278 [new locus tag: NWMN_RS07205 ]
pan locus tag^?: SAUPAN003759000
symbol: NWMN_1278
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1278 [new locus tag: NWMN_RS07205 ]
• symbol: NWMN_1278
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1408869..1409900
• length: 1032
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332575 NCBI
• RefSeq: YP_001332312 NCBI
• BioCyc:
• MicrobesOnline: 3706830 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
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  661
  721
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  841
  901
  961
  1021
  
  ATGGAACCAATATTAGAAATGATTAAAACATTAACAGGTATTAATAGTCCTTCAGGAGAC
  ACAGAAGAAGCAATTCAATTTGTCGAAAAATATGCAAAAGACTTGGGTTATCAAACAACA
  CTAACAAATAAAGGTGCGTTATTAATAACAGTGCCAGGCAAAAATGATGAAGTACAACGC
  TGTATTACTGCTCATGTTGATACTTTAGGTGCAATGGTTAAAGAAATTAAAGAAGATGGT
  CGCTTAGCAATAGAATTAATTGGAGGATTCACGTATAACGCGATTGAGGGTGAATATTGC
  CAAATTAAAACTGATGCTGGTCAAATATATACAGGAACAATTTGTCTGCATGAAACAAGT
  GTTCATGTATATAGAAATAATCATGAAATACCTAGAGATCAAAAGCATATGGAAATAAGA
  ATTGATGAAGTAACTACATCAGAAGAAGATACAAAGAGTTTAGGTATTTCAGTAGGTAAT
  TTTGTTAGCTTTGATCCACGTACAGTTATCACGTCATCAGGTTTTATTAAATCTCGTCAT
  TTAGATGATAAAGCTAGCGTAGCGATGATACTACAATTACTAAAGAAATTAAAAGAAGAG
  CAAATAATATTACCACATACAACGCAATTTTATATTTCTAATAACGAAGAAATAGGTTAC
  GGTGCAAATGCATCAATTGATTCGAAAATCAAAGAATATATTGCATTAGATATGGGCGCG
  TTGGGAGACGGTCAAGCATCGGATGAATATACAGTTTCTATTTGTGCCAAAGATGCTTCA
  GGTCCATATCATAAGCAATTGAAATCGCACCTAGTTAATCTTTGCAAAATAAATAACATT
  CCATATAAAGTAGACATATATCCATATTATGGTTCAGATGCTTCAGCAGCTTTACATGCT
  GGTGCGGATATCAGACATGGTTTATTTGGCGCTGGCATTGAATCATCTCATGCAATGGAA
  CGAACACATATTGATTCTATTAAAGCGACAGAGAAATTACTATATGCATATTGCTTATCA
  CCAATTGAGTAA
  60
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  1032
  

⊟Protein

⊟General

• locus tag: NWMN_1278 [new locus tag: NWMN_RS07205 ]
• symbol: NWMN_1278
• description: hypothetical protein
• length: 343
• theoretical pI: 5.25114
• theoretical MW: 37854.6
• GRAVY: -0.262099

⊟Function

• ⊞⊞TIGRFAM:
  hydrolase, peptidase M42 family (TIGR03106; HMM-score: 328)
  and 5 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides glutamyl aminopeptidase (TIGR03107; EC 3.4.11.7; HMM-score: 72.1)
  peptidase T-like protein (TIGR01883; HMM-score: 19.4)
  N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 17.3)
  Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 16.5)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 15.5)
• TheSEED  :
  • Deblocking aminopeptidase (EC 3.4.11.-)
  Protein Metabolism Protein degradation Protein degradation  Deblocking aminopeptidase (EC 3.4.11.-)
• ⊞⊞PFAM:
  ZnExoPePases (CL0865) Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 296.5)
  and 3 more
  Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 31.1)
  Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 17.6)
  SH3 (CL0010) DUF3247; Protein of unknown function (DUF3247) (PF11607; HMM-score: 16.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: a divalent metal cation
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9683
  • Cytoplasmic Membrane Score: 0.0092
  • Cell wall & surface Score: 0.0006
  • Extracellular Score: 0.0219
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008337
  • TAT(Tat/SPI): 0.000642
  • LIPO(Sec/SPII): 0.000865
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221490 NCBI
• RefSeq: YP_001332312 NCBI
• UniProt: A0A0H3K8J2 UniProt

⊟Protein sequence

• MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNKGALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQIKTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGNFVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEIGYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCKINNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLLYAYCLSPIE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

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⊟Literature

⊟References

⊟Relevant publications