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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0671 [new locus tag: SACOL_RS03470 ]
  • pan locus tag?: SAUPAN002483000
  • symbol: SACOL0671
  • pan gene symbol?: —
  • synonym:
  • product: alpha/beta fold family hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0671 [new locus tag: SACOL_RS03470 ]
  • symbol: SACOL0671
  • product: alpha/beta fold family hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 698937..699725
  • length: 789
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATAAAGTCACAATTAATCCTCAAATCCAATTAACTTATCAAATTGAAGGTAAAGGG
    GATCCTATAATATTACTTCATGGATTGGATGGTAATTTAGCTGGATTTGAAGATTTGCAA
    CATCAACTAGCATCATCATATAAAGTACTTACTTACGATTTAAGAGGTCATGGCAAGTCT
    TCTAAAAGTGAATCATACGATTTAAACGATCACGTTGAGGATTTAAAAATTCTAATGGAG
    AAGTTAAATATTCATGAGGCACATATTCTAGGACATGATTTAGGTGGGGTAGTTGCTAAG
    TTATTTACAGATAAATATGCTTATCGTGTAAAATCATTAACTACCATTGCATCGAAGAAA
    GATGACTTAATACACAGCTTTACTCAATTGTTAATACAATATCAAGATGATATAGCGGGT
    TTTAATAAGTCTGAAGCGTATATTCTTTTATTTTCTAAATTGTTTAGAAATCAAGAGAAG
    ACGATGAAATGGTATCAAAAACAAAGAATATATAGCATTAAGTCTGAGGATGATAGTGCG
    GTGGCAATTCGTTCATTAATTTTGCATAAAGATGAACCTATGTATTTAAAAAAACGTACA
    TGTGTACCTACTTTGTTAATTAATGGGGAACATGATCCTTTGATTAAAGATAAGAATCAT
    TTTAAATTGGAAGCGCATTTTTTAAATGTTACGAAAAAAATCTTCGAACATTCAGGACAT
    GCACCGCATATTGAAGAACCAGAAGCATTTATGAATTATTATTTAAAATTTTTAAAAAGC
    GTATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    789

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0671 [new locus tag: SACOL_RS03470 ]
  • symbol: SACOL0671
  • description: alpha/beta fold family hydrolase
  • length: 262
  • theoretical pI: 7.9427
  • theoretical MW: 30215.5
  • GRAVY: -0.41374

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 78.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 76.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 72.9)
    pyrimidine utilization protein D (TIGR03611; HMM-score: 68.1)
    and 6 more
    proline-specific peptidase (TIGR01250; HMM-score: 59.7)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 47.9)
    prolyl aminopeptidase (TIGR01249; EC 3.4.11.5; HMM-score: 35.3)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 34)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 27.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 13.2)
  • TheSEED  :
    • hypothetical esterase/lipase [EC:3.1.-.-]
  • ⊞PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 112.9)
    and 9 more
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 60.3)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 59.4)
    PGAP1; PGAP1-like alpha/beta domain (PF07819; HMM-score: 21.3)
    FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 21.2)
    Ndr; Ndr family (PF03096; HMM-score: 17.6)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 16.6)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 15.2)
    Abhydrolase_4; TAP-like protein (PF08386; HMM-score: 13.4)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7543
    • Cytoplasmic Membrane Score: 0.0747
    • Cell wall & surface Score: 0.0125
    • Extracellular Score: 0.1585
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007481
    • TAT(Tat/SPI): 0.000325
    • LIPO(Sec/SPII): 0.000767
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLASSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLFTDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEKTMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDKNHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: SigB* (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  5. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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