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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1583 [new locus tag: SA_RS08920 ]
  • pan locus tag?: SAUPAN004448000
  • symbol: rot
  • pan gene symbol?: rot
  • synonym:
  • product: repressor of toxins Rot

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1583 [new locus tag: SA_RS08920 ]
  • symbol: rot
  • product: repressor of toxins Rot
  • replicon: chromosome
  • strand: -
  • coordinates: 1821811..1822272
  • length: 462
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGTTTGTTAATACTTGTATGGTAGCTAAATATGTGATTATTAATTGGGAGATGTTTAGC
    ATGAAAAAAGTAAATAACGACACTGTATTTGGGATTTTGCAATTAGAAACACTTTTGGGT
    GACATTAACTCAATTTTCAGCGAGATTGAAAGCGAATACAAAATGTCTAGAGAAGAAATT
    TTAATTTTACTAACTTTATGGCAAAAAGGTTCTATGACGCTTAAAGAAATGGACAGATTT
    GTTGAAGTTAAACCGTATAAGCGTACGAGAACGTATAATAATTTAGTTGAATTAGAATGG
    ATTTACAAAGAGCGTCCTGTTGACGATGAAAGAACAGTTATTATTCATTTCAATGAAAAG
    TTACAACAAGAGAAAGTAGAGTTGTTGAATTTCATCAGTGATGCCATTGCAAGTAGAGCA
    ACAGCAATGCAAAATAGTTTAAACGCAATTATTGCTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    462

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1583 [new locus tag: SA_RS08920 ]
  • symbol: Rot
  • description: repressor of toxins Rot
  • length: 153
  • theoretical pI: 4.85848
  • theoretical MW: 18008.9
  • GRAVY: -0.0843137

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 76.6)
  • TheSEED  :
    • Repressor of toxins Rot
  • ⊞PFAM:
    HTH (CL0123) Staph_reg_Sar_Rot; Transcriptional regulator SarA/Rot (PF22381; HMM-score: 75.5)
    and 2 more
    MarR; MarR family (PF01047; HMM-score: 35.4)
    FMN-binding (CL0336) DUF447_N; Protein of unknown function (DUF447) N-terminal domain (PF04289; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9321
    • Cytoplasmic Membrane Score: 0.0107
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.057
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003583
    • TAT(Tat/SPI): 0.000065
    • LIPO(Sec/SPII): 0.000662
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFVNTCMVAKYVIINWEMFSMKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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