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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA0806 [new locus tag: SA_RS04595 ]
  • pan locus tag?: SAUPAN003046000
  • symbol: SA0806
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0806 [new locus tag: SA_RS04595 ]
  • symbol: SA0806
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 910963..912117
  • length: 1155
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 1123621 NCBI
  • RefSeq: NP_374067 NCBI
  • BioCyc: see SA_RS04595
  • MicrobesOnline: 103093 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCATTGGACAATTATCGGCGGTGGCATACAGGGAACTGCAATCGCACAAAAACTATTA
    TCAAGCGGATTAACAACAGACCTATTAACAATCATTGACCCACACGAAACTTTTTGCCAA
    AGGTTTAACTCATATACAAATCGAATAGAAATGCCTTATTTAAGATCACCGATTGTACAT
    CACGTACATCCACAACCGTTCCATCTAAAACAATTCGCTAAACAGCACCAATATACAAAT
    GCTTTTTATGGTCCTTATCAACGACCTGAATTGACAATGTTTATGAATCATATTGCACAT
    GCTTCTAAACAATATCAATTAGAGGATTGCTTGGTTCAAGGTTTAGTTCAAACTTTAGAT
    AAACAAGAAGACAAATGGCATATCAAGTTAGAAGATGGACAAATTATCACTACAGATTGC
    GTCGTTATTGCAATAGGTAGTACAAATATTCCGTTTATGCCTGACATTTTAAAAGACAAA
    CAGAATGTAAATCATATCTTCGAGAAAGAACATGATCAAGTAGTATACGATAAGACCGAT
    CATATCGTTGGTAGTGGCATTACTGCTGCACATCTTGCACTTAAATTGTTAAATCATGAT
    AACGATAAAAAGATTCATTTATGGCTAAATAAAGATATTGAAATACATGACTTTGATGCT
    GATCCTGGTTGGTTAGGTCCGAAAAATATGTCTTCATTTTTAAGTACTAAAAGCATGCCT
    GAAAGAAATGCCATTGTACAACGTGAACGTCATAAAGGATCAATGCCTCACGAACTGTAC
    TTACGCCTTAAAAAACATATTAAAAATGGTCGTATAAATGTGCATAAAACACCTATCACT
    CAAATTAGTGGTGGTGTAATTAACACTGAAAATGATTCTGTTCCATATCAACAGATTATG
    GTTGCAACTGGTTTTGAACAAGATTTTATGTCACAACCACTTATTAAGCAATTAATACAA
    AATTATGATGCACCTATCAACGAATGTAATTACCCTGTTATTTCCGAAAAATTAGAATGG
    ATACCAAATCTATTTGTCGCAGGATGCTTTGCAGACTTAGAATTAGGACCATTTGGTAGA
    AATGTTATGGGTGGCCGTAAAGCTGCCGAACGCATTGAACAAGCATTTCTAAAATTACAA
    CAATATAGCGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1155

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0806 [new locus tag: SA_RS04595 ]
  • symbol: SA0806
  • description: hypothetical protein
  • length: 384
  • theoretical pI: 7.10129
  • theoretical MW: 43935
  • GRAVY: -0.400521

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 31.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 25.6)
    and 10 more
    lycopene cyclase family protein (TIGR01790; HMM-score: 22.2)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 19.3)
    putative histamine N-monooxygenase (TIGR04439; EC 1.14.13.-; HMM-score: 17.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 14.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 13.8)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 12.1)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 11.2)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 10.7)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 10.5)
  • TheSEED  :
    • FIG01108091: hypothetical protein
  • ⊞PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 42.7)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 41.9)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 40.5)
    and 8 more
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 24.6)
    no clan defined MptE-like; 6-hydroxymethylpterin diphosphokinase MptE-like (PF01973; HMM-score: 23.2)
    NADP_Rossmann (CL0063) NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 22.4)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.5)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 15.5)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 15)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 14.2)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7611
    • Cytoplasmic Membrane Score: 0.2212
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0175
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.027346
    • TAT(Tat/SPI): 0.001354
    • LIPO(Sec/SPII): 0.011394
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 15926534 NCBI
  • RefSeq: NP_374067 NCBI
  • UniProt: A0A0H3JLD5 UniProt

⊟Protein sequence[edit | edit source]

  • MHWTIIGGGIQGTAIAQKLLSSGLTTDLLTIIDPHETFCQRFNSYTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMNHIAHASKQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDKQNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDFDADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHKTPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVISEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • ⊞regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA0806&oldid=75419"
  • This page was last edited on 10 March 2016, at 23:23.
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