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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12690 [old locus tag: SA2211 ]
  • pan locus tag?: SAUPAN005963000
  • symbol: SA_RS12690
  • pan gene symbol?: bioW
  • synonym:
  • product: 6-carboxyhexanoate--CoA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12690 [old locus tag: SA2211 ]
  • symbol: SA_RS12690
  • product: 6-carboxyhexanoate--CoA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2481878..2482570
  • length: 693
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2481878..2482570) NCBI
  • BioCyc: SA_RS12690 BioCyc
  • MicrobesOnline: see SA2211

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTATAGTATAAAAATGCGTTCTAGCAATCAAGATGTTCATATTAGTGGTGCTGAAACG
    ATTTGTGAGTTTGACAAAATAGAACAGACGGTACAAAGGTTTTATAATAAAGGTTTTTTT
    CATGAAAATGGGCAACCGGATTTTCTCAATATAAAAATACAAAAAATTATGGAACCGATT
    CAACAAATAAAAGCATTACAAATTATTGAGGATGATAAAGCAAATCTCCAACATTTAACA
    CAAGAATGTGGTGTCACTGAACAAGCGCTAAATCAAGGGATGACATATATTAAAAATGAA
    ACGGTTTATACAGGAGCTATTATTCTATCTGCTATATCTGGTAAACGTTTAGATTCATTT
    GGACAAAGGGGGATTAGGGCAACACATTTTTCGTTTGAAGATATAAATAATAAAGGTGAT
    CTTAATGAAAGAGTGACTGATGCCTTAGCAATTGCTAGTTGTATCAATGCGCATCCGTAT
    GTCAAAGGAGAACTTTGCGTGTCCGATGACTTAACGTATACGACAGGTTATTTTGCCGCT
    GCTAAAATTGGTTACCATCGATTATTTGATATTAAACCAGTTAATACGAGATATGGAGGC
    AGAATAATATTTGTGGACGATTGTATTGATTTAAATCATTACATATCATTTTTAGAAAGC
    ACACCGAAGCAAGTTGTTTATGAAACGGTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12690 [old locus tag: SA2211 ]
  • symbol: SA_RS12690
  • description: 6-carboxyhexanoate--CoA ligase
  • length: 230
  • theoretical pI: 5.88586
  • theoretical MW: 26091.4
  • GRAVY: -0.29087

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.2.1.14?  ExPASy
    6-carboxyhexanoate--CoA ligase ATP + 6-carboxyhexanoate + CoA = AMP + diphosphate + 6-carboxyhexanoyl-CoA
  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 6-carboxyhexanoate--CoA ligase (TIGR01204; EC 6.2.1.14; HMM-score: 155.1)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase (TIGR01498; EC 2.7.6.3; HMM-score: 12.5)
  • TheSEED: see SA2211
  • ⊞PFAM:
    no clan defined BioW; 6-carboxyhexanoate--CoA ligase (PF03744; HMM-score: 253.2)
    and 1 more
    eIF3_N; eIF3 subunit 6 N terminal domain (PF09440; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.4592
    • Cytoplasmic Membrane Score: 0.0081
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.5323
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002872
    • TAT(Tat/SPI): 0.000254
    • LIPO(Sec/SPII): 0.000549
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENGQPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETVYTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPYVKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFLESTPKQVVYETV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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