PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0032 [new locus tag: SA_RS00305 ]
pan locus tag^?: SAUPAN000091000
symbol: bleO
pan gene symbol^?: bleO
• synonym:
• product: bleomycin resistance protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0032 [new locus tag: SA_RS00305 ]
• symbol: bleO
• product: bleomycin resistance protein
• replicon: chromosome
• strand: -
• coordinates: 40197..40601
• length: 405
• essential: no DEG

⊟Accession numbers

• Gene ID: 1122806 NCBI
• RefSeq: NP_373272 NCBI
• BioCyc: see SA_RS00305
• MicrobesOnline: 102298 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  GTGAGAATGTTACAGTCTATCCCGGCATTGCCAGTCGGGGATATTAAAAAGAGTATAGGT
  TTTTATTGCGATAAACTAGGTTTCACTTTGGTTCACCATGAAGATGGATTCGCAGTTCTA
  ATGTGTAATGAGGTTCGGATTCATCTATGGGAGGCAAGTGATGAAGGCTGGCGCTCTCGT
  AGTAATGATTCACCGGTTTGTACAGGTGCGGAGTCGTTTATTGCTGGTACTGCTAGTTGC
  CGCATTGAAGTAGAGGGAATTGATGAATTATATCAACATATTAAGCCTTTGGGCATTTTG
  CACCCCAATACATCATTAAAAGATCAGTGGTGGGATGAACGAGACTTTGCAGTAATTGAT
  CCCGACAACAATTTGATTAGCTTTTTTCAACAAATAAAAAGCTAA
  60
  120
  180
  240
  300
  360
  405
  

⊟Protein

⊟General

• locus tag: SA0032 [new locus tag: SA_RS00305 ]
• symbol: BleO
• description: bleomycin resistance protein
• length: 134
• theoretical pI: 4.74765
• theoretical MW: 15209.2
• GRAVY: -0.218657

⊟Function

• ⊞TIGRFAM:
  Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 16.9)
  Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 16.9)
  and 1 more
  3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 11.5)
• TheSEED  :
  • Bleomycin-binding protein
• ⊞PFAM:
  Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 50.6)
  and 5 more
  Ble-like_N; Bleomycin resistance protein-like N-terminal (PF22677; HMM-score: 24.7)
  Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 19.3)
  Glyoxalase_7; Glyoxalase superfamily protein (PF19581; HMM-score: 15.8)
  CppA_N; CppA N-terminal (PF14506; HMM-score: 13.5)
  YycE-like_N; YycE-like N-terminal domain (PF22658; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9887
  • Cytoplasmic Membrane Score: 0.0011
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0102
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014668
  • TAT(Tat/SPI): 0.00044
  • LIPO(Sec/SPII): 0.001316
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925739 NCBI
• RefSeq: NP_373272 NCBI
• UniProt: Q7A8D1 UniProt

⊟Protein sequence

• MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVIDPDNNLISFFQQIKS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)