PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2610 [new locus tag: NWMN_RS14945 ]
pan locus tag^?: SAUPAN006487000
symbol: gidB
pan gene symbol^?: gidB
• synonym:
• product: 16S rRNA methyltransferase GidB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2610 [new locus tag: NWMN_RS14945 ]
• symbol: gidB
• product: 16S rRNA methyltransferase GidB
• replicon: chromosome
• strand: -
• coordinates: 2873891..2874610
• length: 720
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331629 NCBI
• RefSeq: YP_001333644 NCBI
• BioCyc:
• MicrobesOnline: 3708212 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTGTAGAATGGTTAGCAGAACAATTAAAAGAACATAATATTCAATTAACTGAGACT
  CAAAAACAACAGTTTCAAACATATTATCGTTTACTTGTTGAATGGAATGAAAAGATGAAT
  TTGACAAGTATTACAGATGAACACGATGTATATTTGAAACATTTTTATGATTCCATTGCA
  CCTAGTTTTTATTTTGATTTTAATCAGCCTATAAGTATATGTGATGTAGGCGCTGGAGCT
  GGTTTTCCAAGTATTCCGTTAAAAATAATGTTTCCGCAGTTAAAAGTGACGATTGTTGAT
  TCATTAAATAAGCGTATTCAATTTTTAAACCATTTAGCGTCAGAATTACAATTACAGGAT
  GTCAGCTTTATACACGATAGAGCAGAAACATTTGGTAAGGGTGTCTACAGGGAGTCTTAT
  GATGTTGTTACTGCAAGAGCAGTAGCTAGATTATCCGTGTTAAGTGAATTGTGTTTACCG
  CTAGTTAAAAAAGGTGGACAGTTTGTTGCATTAAAATCTTCAAAAGGTGAAGAAGAATTA
  GAAGAAGCAAAATTTGCAATTAGTGTGTTAGGTGGTAATGTTACAGAAACACATACCTTT
  GAATTGCCAGAAGATGCTGGAGAGCGCCAGATGTTCATTATTGATAAAAAAAGACAGACG
  CCGAAAAAGTATCCAAGAAAACCAGGGACGCCTAATAAGACTCCTTTACTTGAAAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_2610 [new locus tag: NWMN_RS14945 ]
• symbol: GidB
• description: 16S rRNA methyltransferase GidB
• length: 239
• theoretical pI: 6.52152
• theoretical MW: 27359.1
• GRAVY: -0.37113

⊟Function

• reaction:
  EC 2.1.1.-?  ExPASy
• ⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 174.9)
  and 7 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 28.9)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 22.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 20.2)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 17.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll C-20 methyltransferase BchU (TIGR02716; EC 2.1.1.-; HMM-score: 16)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 14.1)
• TheSEED  :
  • 16S rRNA (guanine(527)-N(7))-methyltransferase (EC 2.1.1.170)
  Cell Division Subsystem including YidCD  rRNA small subunit methyltransferase, glucose inhibited division protein GidB
• ⊞PFAM:
  NADP_Rossmann (CL0063) GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 192.7)
  and 6 more
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 38.2)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 26.4)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 19.2)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 17.2)
  Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 15.1)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9977
  • Cytoplasmic Membrane Score: 0.002
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0001
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001895
  • TAT(Tat/SPI): 0.000205
  • LIPO(Sec/SPII): 0.000499
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222822 NCBI
• RefSeq: YP_001333644 NCBI
• UniProt: A6QKK0 UniProt

⊟Protein sequence

• MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSITDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSLNKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLPLVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKRQTPKKYPRKPGTPNKTPLLEK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2609 < gidB < gidA < trmE

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications