PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_01271
pan locus tag^?: SAUPAN003598000
symbol: SAOUHSC_01271
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_01271
• symbol: SAOUHSC_01271
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1225887..1226378
• length: 492
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3919924 NCBI
• RefSeq: YP_499804 NCBI
• BioCyc: G1I0R-1189 BioCyc
• MicrobesOnline: 1289718 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGAAATCAAGAAAACTGGCTATAACTGCACTTTTAATTGCAATAAATGTTGTATTAAGC
  AGTATTATCATCATTCCTCTAGGACCAGTTAAGGCAGCACCAGTACAGCATTTTGTAAAT
  GTATTAAGTGCGGTCATAGTAGGTCCTTGGTATGGATTAGCTCAAGCGCTTATATCATCA
  ATTTTAAGAGTTCTTTTTGGTACTGGTACAGCTTTTGCATTTCCGGGTAGTATGATTGGA
  GTTTTATTGGCTAGTATGTTTTACATATATCGTAAACATATATTTATGGCCGCGGTCGGT
  GAAGTACTTGGAACTGGTGTCATCGGAAGTTTAATTTGTATACCATTAGCATATTTCCTT
  GGGCTTCAAGACTTCTTCATTAAACCGTTAATGATTACGTTCATAGTCTCAAGTGCTATC
  GGATCTATTATAAGTTATTTCTTATTAATTACTCTAAAAAAACGTGGTATTCTTCAAAGG
  TTTATAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  492
  

⊟Protein

⊟General

• locus tag: SAOUHSC_01271
• symbol: SAOUHSC_01271
• description: hypothetical protein
• length: 163
• theoretical pI: 10.9181
• theoretical MW: 17600.5
• GRAVY: 1.23067

⊟Function

• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
  Metabolism Transport and binding proteins Other energy coupling factor transporter S component ThiW (TIGR02359; HMM-score: 207)
  and 2 more
  Metabolism Transport and binding proteins Unknown substrate ECF transporter S component, folate family (TIGR04518; HMM-score: 36.3)
  Cell structure Cell envelope Surface structures PEP-CTERM protein-sorting domain (TIGR02595; HMM-score: 9)
• ⊞TheSEED  :
  • Substrate-specific component ThiW of predicted thiazole ECF transporter
  Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Substrate-specific component ThiW of predicted thiazole ECF transporter
  and 1 more
  Membrane Transport Membrane Transport - no subcategory ECF class transporters  Substrate-specific component ThiW of predicted thiazole ECF transporter
• ⊞PFAM:
  Gx_transp (CL0315) ThiW; Thiamine-precursor transporter protein (ThiW) (PF09512; HMM-score: 185.9)
  and 2 more
  ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 46.1)
  ECF-ribofla_trS; ECF-type riboflavin transporter, S component (PF07155; HMM-score: 25.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0002
  • Cytoplasmic Membrane Score: 0.9991
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0007
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: Signal peptide SP(Sec/SPI) length 32 aa
  • SP(Sec/SPI): 0.879749
  • TAT(Tat/SPI): 0.014507
  • LIPO(Sec/SPII): 0.003793
  • Cleavage Site: CS pos: 32-33. VKA-AP. Pr: 0.8741
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 88195004 NCBI
• RefSeq: YP_499804 NCBI
• UniProt: Q2FZ00 UniProt
• STRING: 93061.SAOUHSC_01271 STRING

⊟Protein sequence

• MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSAVIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEVLGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQRFIK

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_01269 > SAOUHSC_01270 > SAOUHSC_01271
  predicted SigA promoter  : SAOUHSC_01266 > SAOUHSC_01267 > S529 > SAOUHSC_01268 > S530 > SAOUHSC_01269 > SAOUHSC_01270 > SAOUHSC_01271
  predicted SigA promoter  : SAOUHSC_01269 > SAOUHSC_01270 > SAOUHSC_01271 > S531 > SAOUHSC_01272

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications