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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13580 [old locus tag: SACOL2593 ]
  • pan locus tag?: SAUPAN006252000
  • symbol: SACOL_RS13580
  • pan gene symbol?: gbaA
  • synonym: rob
  • product: TetR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS13580 [old locus tag: SACOL2593 ]
  • symbol: SACOL_RS13580
  • product: TetR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2655057..2655614
  • length: 558
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (2655057..2655614) NCBI
  • BioCyc: SACOL_RS13580 BioCyc
  • MicrobesOnline: see SACOL2593

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCGAAAAGATGCAAAAGAGAATAGGCAACGAATTGAAGAAATAGCGCACAAACTTTTT
    GATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGT
    ATGGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAA
    AGAGACCTTGATATTTTTATCACTCATTTTAAGCAAATTAAAGATGACTATCATTCTAAT
    TATGAGGTTATGCAAGTGTCGTTAGATTATCTTTTACAATTTAAAATCGATAATAAGGCA
    CTACTGCAATGTATTGAAGCAGGCAACAATAAATTACGTTTTTACCAAAGCGCATTTTAT
    CAAGAATTATTTGATTTTTATTATGACTTATTTAAAAGCGATGACGACACTTACACGAAG
    TTCAAAACAGATATGCTGTTGCAATCATTATCAACTAGTGTATTTGCCTTCCAAATAGAG
    CATCGCCATATTTCAATTGAAGCGTACAGAAATTATTTATTAAACATTTATTTAGATGAG
    GTGGGACGTAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS13580 [old locus tag: SACOL2593 ]
  • symbol: SACOL_RS13580
  • description: TetR family transcriptional regulator
  • length: 185
  • theoretical pI: 5.33099
  • theoretical MW: 22260
  • GRAVY: -0.54

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 41.8)
    and 5 more
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 20.7)
    probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 20.6)
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 16)
    probable regulatory domain (TIGR03879; HMM-score: 13.5)
    Genetic information processing Mobile and extrachromosomal element functions Other probable addiction module antidote protein (TIGR02684; HMM-score: 12.6)
  • TheSEED: see SACOL2593
  • ⊞PFAM:
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 45.6)
    and 6 more
    CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 16.6)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 15.1)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.1)
    4H_Cytokine (CL0053) IL12; Interleukin-12 alpha subunit (PF03039; HMM-score: 12.6)
    HTH (CL0123) HTH_8; Bacterial regulatory protein, Fis family (PF02954; HMM-score: 11.8)
    TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.971
    • Cytoplasmic Membrane Score: 0.014
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0148
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007128
    • TAT(Tat/SPI): 0.000387
    • LIPO(Sec/SPII): 0.001252
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 447146932 NCBI
  • RefSeq: WP_001224188 NCBI
  • UniProt: see SACOL2593

⊟Protein sequence[edit | edit source]

  • MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTLYRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALLQCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIEHRHISIEAYRNYLLNIYLDEVGRND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL2593
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS13580&oldid=91783"
  • This page was last edited on 11 March 2016, at 08:36.
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