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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • pan locus tag?: SAUPAN002586000
  • symbol: nagA
  • pan gene symbol?: nagA
  • synonym:
  • product: N-acetylglucosamine-6-phosphate deacetylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • symbol: nagA
  • product: N-acetylglucosamine-6-phosphate deacetylase
  • replicon: chromosome
  • strand: +
  • coordinates: 750281..751462
  • length: 1182
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTCAGAATTAATTATATATAACGGCAAAGTTTATACTGAAGATGGCAAAATCGATAAT
    GGTTACATTCATGTGAAAGATGGACAGATTGTTGCAATTGGAGAAGGGGATGATAAAGCA
    GCAATTGATAATGATACGACAAATAAAATTCAAGTGATTGATGCTAAAGGTCATCATGTA
    TTACCAGGTTTTATTGATATACATATTCATGGTGGTTATGGTCAAGATGCAATGGATGGG
    TCATACGATGGCTTAAAATATCTATCCGAAAATTTGTTATCTGAAGGGACGACATCATAC
    TTGGCCACTACAATGACGCAATCTACTGATAAAATAGATAAAGCACTTACAAATATTGCT
    AAATATGAAGCGGAGCAAGATGTTCACAATGCAGCGGAAATTGTAGGTATACATTTAGAA
    GGACCATTTATATCTGAAAATAAAGTTGGTGCTCAACATCCGCAATACGTTGTACGCCCA
    TTTATCGATAAAATTAAACATTTTCAAGAGACTGCTAACAGATTAATAAAGATTATGACG
    TTTGCACCTGAAGTTGAAGGTGCAAAAGAAGCGCTTGAAACGTATAAAGATGACATTATT
    TTTTCAATTGGTCATACAGTGGCAACATACGAAGAAGCAGTCGAAGCTGTTGAGCGAGGA
    GCTAAACATGTCACGCATTTATATAATGCAGCGACGCCATTCCAACATAGAGAACCAGGT
    GTTTTTGGAGCAGCATGGTTGAATGATGCTCTACATACCGAAATGATTGTTGATGGCACA
    CATTCTCATCCGGCATCGGTTGCAATTGCTTACCGTATGAAAGGTAATGAACGTTTTTAT
    TTAATTACCGATGCAATGCGTGCAAAAGGTATGCCTGAAGGAGAATATGATTTGGGTGGA
    CAAAAAGTAACTGTTCAATCGCAACAAGCACGTCTTGCAAATGGTGCGCTTGCTGGTAGT
    ATTTTAAAAATGAATCATGGGTTACGTAACTTAATATCATTTACAGGTGATACATTAGAT
    CATTTATGGCGAGTAACAAGTTTAAATCAAGCCATTGCATTAGGTATCGATGATAGAAAA
    GGTAGTATTAAAGTAAATAAGGATGCAGATCTTGTTATTCTAGATGATGATATGAATGTA
    AAATCTACAATAAAACAAGGCAAGGTTCACACATTTAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0656 [new locus tag: SA_RS03745 ]
  • symbol: NagA
  • description: N-acetylglucosamine-6-phosphate deacetylase
  • length: 393
  • theoretical pI: 5.76193
  • theoretical MW: 43186.3
  • GRAVY: -0.325954

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 376.8)
    and 12 more
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 45.9)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 44.3)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 41.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 41.1)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 41)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 35.2)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 35.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 34.1)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 26.1)
    Metabolism Central intermediary metabolism Nitrogen metabolism urease, alpha subunit (TIGR01792; EC 3.5.1.5; HMM-score: 20.8)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 18.6)
    Metabolism Energy metabolism Amino acids and amines formiminoglutamate deiminase (TIGR02022; EC 3.5.3.13; HMM-score: 15.4)
  • ⊞TheSEED  :
    • N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    and 2 more
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
  • ⊞PFAM:
    TIM_barrel (CL0036) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 93.2)
    and 2 more
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 55.8)
    BsNagA_N; BsNagA composite domain (PF22644; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9982
    • Cytoplasmic Membrane Score: 0.0008
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0008
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007019
    • TAT(Tat/SPI): 0.000217
    • LIPO(Sec/SPII): 0.001092
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDNDTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLATTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRPFIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVERGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGNERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISFTGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTFS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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