PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary

• organism: Staphylococcus aureus NCTC8325
locus tag: SAOUHSC_02999
pan locus tag^?: SAUPAN006408000
symbol: SAOUHSC_02999
pan gene symbol^?: cap1B
• synonym:
• product: capsular polysaccharide biosynthesis protein Cap5B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAOUHSC_02999
• symbol: SAOUHSC_02999
• product: capsular polysaccharide biosynthesis protein Cap5B
• replicon: chromosome
• strand: -
• coordinates: 2772230..2772922
• length: 693
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3921481 NCBI
• RefSeq: YP_501448 NCBI
• BioCyc: G1I0R-2821 BioCyc
• MicrobesOnline: 1291419 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACGAATACACGAAGAAGTACATCAAGTTTAATTGTCCATGAACAACCAAAGTCACCT
  ATTAGCGAGAAATTTCGAGGCATAAGATCAAATATTATGTTTGCAAATCCTGACAGTGCA
  GTTCAAAGCATTGTAATCACTTCAGAGGCACCAGGCGCAGGTAAGTCTACAATTGCAGCA
  AATTTAGCAGTTGCATATGCGCAAGCAGGTTATAAAACACTAATCGTAGACGGGGATATG
  CGTAAACCTACGCAGCATTATATTTTTAATTTGCCAAACAATGAAGGCCTATCAAGTTTA
  TTGCTAAATTGGTCAACTTATCAAGACAGTATTATCTCAACTGAAATTCAAGATTTAGAC
  GTCTTGACGTCTGGGCCAATCCCACCGAATCCGTCAGAGTTAATTACATCAAGGGCATTT
  GCAAATTTGTATGACACATTATTGATGAATTATAACTTTGTAATTATCGATACGCCACCA
  GTGAACACAGTTACAGATGCGCAATTATTTTCAAAGTTTACCGGCAATGTTGTCTACGTA
  GTTAATTCGGAAAATAATAATAGAGATGAAGTTAAAAAAGGAAAAGAACTTATTGAAGCA
  ACAGGTGCTAAATTATTAGGTGTAGTCTTAAATAGAATGCCTAAAGATAAAAGTGCTAGT
  TACTATGCATATTATGGGACTGATGAATCATGA
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  693
  

⊟Protein

⊟General

• locus tag: SAOUHSC_02999
• symbol: SAOUHSC_02999
• description: capsular polysaccharide biosynthesis protein Cap5B
• length: 230
• theoretical pI: 5.30455
• theoretical MW: 25251.3
• GRAVY: -0.247391

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids capsular exopolysaccharide family (TIGR01007; HMM-score: 258.7)
  and 17 more
  chain length determinant protein tyrosine kinase EpsG (TIGR03029; HMM-score: 145.5)
  exopolysaccharide/PEP-CTERM locus tyrosine autokinase (TIGR03018; EC 2.7.10.2; HMM-score: 129.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids exopolysaccharide transport protein family (TIGR01005; HMM-score: 113.1)
  cell division ATPase MinD (TIGR01969; HMM-score: 55.5)
  Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 40.2)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjQ (TIGR03371; HMM-score: 37.4)
  helicase/secretion neighborhood CpaE-like protein (TIGR03815; HMM-score: 25.7)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase iron protein (TIGR01287; EC 1.18.6.1; HMM-score: 17.4)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions plasmid partitioning protein RepA (TIGR03453; HMM-score: 17.4)
  Hypothetical proteins Conserved transport-energizing ATPase, TRC40/GET3/ArsA family (TIGR00345; EC 3.6.1.-; HMM-score: 16.7)
  arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16.3)
  Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 16.2)
  putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 14.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-independent protochlorophyllide reductase, iron-sulfur ATP-binding protein (TIGR01281; EC 1.3.7.7; HMM-score: 13.8)
  signal recognition particle protein SRP54 (TIGR01425; HMM-score: 12.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 11.7)
  HprK-related kinase A (TIGR04352; HMM-score: 10.9)
• ⊞TheSEED  :
  • Capsular polysaccharide synthesis enzyme Cap5B
  • Tyrosine-protein kinase EpsD (EC 2.7.10.2)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Exopolysaccharide Biosynthesis  Tyrosine-protein kinase EpsD (EC 2.7.10.2)
  and 1 more
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Tyrosine-protein kinase EpsD (EC 2.7.10.2)
• ⊞PFAM:
  P-loop_NTPase (CL0023) AAA_31; AAA domain (PF13614; HMM-score: 49.8)
  ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 46.1)
  CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 45.9)
  and 14 more
  MipZ; ATPase MipZ (PF09140; HMM-score: 35.6)
  AAA_26; AAA domain (PF13500; HMM-score: 27.3)
  nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 23.9)
  CBP_BcsQ; Cellulose biosynthesis protein BcsQ (PF06564; HMM-score: 21.8)
  ArsA_ATPase; Anion-transporting ATPase (PF02374; HMM-score: 19.4)
  nSTAND_NTPase7; Novel STAND NTPase 7 (PF24404; HMM-score: 18.9)
  AAA_25; AAA domain (PF13481; HMM-score: 17.9)
  CLP1_P; mRNA cleavage and polyadenylation factor CLP1 P-loop (PF16575; HMM-score: 17.5)
  APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 16.3)
  Fer4_NifH; 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family (PF00142; HMM-score: 14.9)
  SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 14.4)
  MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 12.1)
  TIM_barrel (CL0036) OAM_alpha; D-ornithine 4,5-aminomutase alpha-subunit (PF16552; HMM-score: 12)
  P-loop_NTPase (CL0023) KTI12; Chromatin associated protein KTI12 (PF08433; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7061
  • Cytoplasmic Membrane Score: 0.2636
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0296
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024914
  • TAT(Tat/SPI): 0.011712
  • LIPO(Sec/SPII): 0.002336
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 88196618 NCBI
• RefSeq: YP_501448 NCBI
• UniProt: Q2FUV2 UniProt
• STRING: 93061.SAOUHSC_02999 STRING

⊟Protein sequence

• MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSIVITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLLNWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPPVNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKSASYYAYYGTDES

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAOUHSC_02998 < SAOUHSC_02999 < SAOUHSC_03000

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser:   
  
  ⊟Multi-gene expression profiles

  Compare with:

  Conditions

  Select all flucRPMI-exp RPMI-exp-A2 vancRPMI-exp RPMI-exp-A1 TSB-exp-A TSB-exp-C ciproTSB-exp T/SmxTSB-exp clindTSB-exp lineTSB-exp eryTSB-exp TSB-exp-B3 flucTSB-exp TSB-exp-B2 vancTSB-exp TSB-exp-B1 RPMI/FCS-exp colist-exp intTHP1-2h intTHP1-6h intS9-6h intS9-2h TSB-t2-C TSB-t4-C flucTSB-t4 TSB-t2-B1 TSB-t4-B1 CDM-t2 CDM-t4 T/SmxTSB-t4 TSB-t4-B3 lineTSB-t4 TSB-t4-B2 vancTSB-t4 eryTSB-t4 clindTSB-t4 ciproTSB-t4 pMEM-t2-C1 pMEM-t4-C1 CO2-6h CO2-2h anaer-2h nonad50-1h CO2-1h nonad25-1h pMEM-exp-C2 pMEM-exp-C1 CDM-exp plasma-t4 vancRPMI-t4 flucRPMI-t4 RPMI-t4-A2 RPMI-t2-A1 plasma-exp

  
  
  Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications