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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01069
  • pan locus tag?: SAUPAN003346000
  • symbol: SAOUHSC_01069
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01069
  • symbol: SAOUHSC_01069
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1035477..1036514
  • length: 1038
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3921731 NCBI
  • RefSeq: YP_499615 NCBI
  • BioCyc: G1I0R-1005 BioCyc
  • MicrobesOnline: 1289528 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAGTTAATTATAAGAGTCGTCGGTGTATTATTTTTAGTTGGATTTTTAATATAT
    CTTTTTTATTCACCGAGATTAAAATTTGATGTATTAGAGAACCCGAATAAAGGTAATAAA
    GTAAATAGATCTGAACAGGTGAATAAATCAAATAACCATGCTGAAAATCCAAAGCCTAAA
    GAAGGTGTTGGTACATGGGTAGGTAAAGATATTAAAGTGCTTACTTCTAAATTTGGACAA
    GCAGATCGTGTCTACCCTTTTAGAGATGGTTACAAAAATTATGTGTTTAAAGACAAAAAC
    AGTTATTACATTGTTTCAACTAAACGTGAAGAAATCGTTTCAGTGTATGCTACAGGTGAG
    AAAGTCAATGTTAGTCCGTTAAAAATAGGACAACATTCTGCAGAAATTTTTAATCATACA
    AGTATTAATCCAGAACCGTCCTTTAAAGTTGATGGTAAAAAATATGAATTTGAACTTTCA
    GATGAAGATTTAAAAACACAAACACTGATTAAATATGGCGACATATATGCACAAGTGTAT
    TCTGATCAACAATCGAAAAAGGTGCTAAGTGTACGATTTTTAACAAAAGAAATGTTAGCA
    GATATTGAACCTTATCAATTAAATTCTAATTCTACGTCAGAAGAGCATAATAAGCGTCCA
    GTTGAGCAAAATCCAAATCAATTAATTTCTCTTTATGAAGTAACGAATGAAATGAGAAAA
    TTAAAAGGATTAAAACCATTGAAAATCAATAGCGATTTAGCACATATTGCATCTAATAAC
    TTATATGAAGCGACCTCTAATGGTTCTGATAGTGTTGAATTTACAGAGGACGCATTAAGA
    GGGCAATTAGATAAAAATCACGTTACTTATAAAACAACTGCTCAAAATGTTGGTTATGCG
    TTTAATGATGTACCAACATTAATCCATAGTTGGATGAATTCAGATATACATCGATCTCGT
    CTATTAAATTCAAAATACGATGAGATGGGTGGAGATGTAATGAGAGATTATTATTCACTA
    ATTTTCTTAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01069
  • symbol: SAOUHSC_01069
  • description: hypothetical protein
  • length: 345
  • theoretical pI: 9.05924
  • theoretical MW: 39612.5
  • GRAVY: -0.626377

⊟Function[edit | edit source]

  • TIGRFAM:
    uncharacterized protein, YkwD family (TIGR02909; HMM-score: 44.8)
  • TheSEED  :
    • Allergen V5/Tpx-1 related
  • ⊞PFAM:
    no clan defined CAP_assoc_N; CAP-associated N-terminal (PF14504; HMM-score: 137.9)
    and 3 more
    CAP (CL0659) CAP; Cysteine-rich secretory protein family (PF00188; HMM-score: 49.4)
    no clan defined Lsr2; Lsr2 dimerization domain (PF11774; HMM-score: 14.2)
    E-set (CL0159) Ig_NUP210_9th; NUP210, Ig-like domain 9 (PF24902; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0008
    • Cytoplasmic Membrane Score: 0.9387
    • Cell wall & surface Score: 0.001
    • Extracellular Score: 0.0595
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.261702
    • TAT(Tat/SPI): 0.001063
    • LIPO(Sec/SPII): 0.015505
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI: 542123268 NCBI
  • RefSeq: YP_499615 NCBI
  • UniProt: Q2FZG1 UniProt
  • STRING: 93061.SAOUHSC_01069 STRING

⊟Protein sequence[edit | edit source]

  • MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRSEQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELSDEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNKRPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTEDALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVMRDYYSLIFLEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SAOUHSC_01069 > SAOUHSC_01070
    predicted SigA promoter [3] : SAOUHSC_01066 > SAOUHSC_01067 > S439 > SAOUHSC_01068 > SAOUHSC_01069 > SAOUHSC_01070
    predicted SigA promoter [3] : SAOUHSC_01068 > SAOUHSC_01069 > SAOUHSC_01070

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [3] 
    Expression Data Browser
    ⊟Multi-gene expression profiles



    Click on any data point to display a description of the corresponding condition!

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Jump up to: 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_01069&oldid=74335"
  • This page was last edited on 10 March 2016, at 22:46.
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