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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08260 [old locus tag: SACOL1620 ]
  • pan locus tag?: SAUPAN004144000
  • symbol: SACOL_RS08260
  • pan gene symbol?: —
  • synonym:
  • product: phosphoenolpyruvate synthetase regulatory protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08260 [old locus tag: SACOL1620 ]
  • symbol: SACOL_RS08260
  • product: phosphoenolpyruvate synthetase regulatory protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1651649..1652467
  • length: 819
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1651649..1652467) NCBI
  • BioCyc: SACOL_RS08260 BioCyc
  • MicrobesOnline: see SACOL1620

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAAAAATTAAAATTATCGTAGCTTCAGATTCTATAGGTGAAACGGCAGAGTTAGTT
    GCTAGGGCAGGTATTTCACAATTCAATCCTAAGCAATGTAAAAATGAATTATTAAGATAT
    CCATATATTGAATCTTTTGAAGATGTTGATGAAGTGATTCAAGTTGCAAAAGATACAAAT
    GCTATCATTGTTTATACACTTATTAAACCTGAAATGAAGCAATATATGAGTGAGAAAGTA
    GCAGAATTCCAATTGAAGTCTGTCGATATCATGGGGCCATTAATGGATTTATTATCTGCT
    TCGGTTGAAGAAAAACCTTATAATGAGCCAGGTATCGTTCATAGATTAGATGATGCATAT
    TTCAAGAAAATTGATGCGATAGAGTTTGCAGTTAAATATGATGATGGTAAAGATCCTAAA
    GGATTACCTAAAGCTGATATTGTTTTACTTGGTATTTCGAGAACTTCAAAGACACCATTA
    TCTCAGTATTTAGCGCATAAGAGTTACAAAGTTATGAATGTACCGATTGTACCAGAAGTG
    ACACCGCCAGATGGCTTATATGATATTAATCCAAAGAAATGTATCGCACTTAAAATAAGT
    GAAGAAAAATTAAATCGCATTAGAAAAGAGCGACTAAAACAATTAGGACTAGGTGACACA
    GCTCGATATGCAACAGAAGCACGAATTCAAGAAGAATTGAATTACTTTGAAGAAATCGTA
    AGTGAAATTGGATGTCCTGTCATTGATGTTTCTCAAAAAGCAATCGAAGAAACAGCAAAC
    GATATAATCCATTATATTGAACAAAATAAATCGAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    819

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08260 [old locus tag: SACOL1620 ]
  • symbol: SACOL_RS08260
  • description: phosphoenolpyruvate synthetase regulatory protein
  • length: 272
  • theoretical pI: 4.92811
  • theoretical MW: 30784.3
  • GRAVY: -0.335294

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.4.27?  ExPASy
    ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase [Pyruvate, phosphate dikinase] phosphate + phosphate = [pyruvate, phosphate dikinase] + diphosphate
    EC 2.7.11.32?  ExPASy
    [Pyruvate, phosphate dikinase] kinase ADP + [pyruvate, phosphate dikinase] = AMP + [pyruvate, phosphate dikinase] phosphate
  • TIGRFAM:
  • TheSEED: see SACOL1620
  • ⊞PFAM:
    P-loop_NTPase (CL0023) Kinase-PPPase; Kinase/pyrophosphorylase (PF03618; HMM-score: 323.9)
    and 3 more
    GlnB-like (CL0089) Nit_Regul_Hom; Uncharacterized protein, homolog of nitrogen regulatory protein PII (PF10126; HMM-score: 13.5)
    HHH (CL0198) DUF655; Protein of unknown function (DUF655) (PF04919; HMM-score: 13.4)
    HTH (CL0123) CDT1; DNA replication factor CDT1 like (PF08839; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9623
    • Cytoplasmic Membrane Score: 0.0152
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0223
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009993
    • TAT(Tat/SPI): 0.000714
    • LIPO(Sec/SPII): 0.001598
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIESFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASVEEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPLSQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLGDTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1620
  • protein localization: see SACOL1620
  • quantitative data / protein copy number per cell: see SACOL1620
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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