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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05370 [old locus tag: SACOL1053 ]
  • pan locus tag?: SAUPAN003247000
  • symbol: SACOL_RS05370
  • pan gene symbol?: menH
  • synonym:
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05370 [old locus tag: SACOL1053 ]
  • symbol: SACOL_RS05370
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1059530..1060333
  • length: 804
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1059530..1060333) NCBI
  • BioCyc: SACOL_RS05370 BioCyc
  • MicrobesOnline: see SACOL1053

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACACATTATAAATTTTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTTCTG
    CATGGATTTCTTAGCGACAGCCGTACTTATCATAATCACATCGAAAAATTTACTGATAAC
    TATCATGTCATCACTATAGACTTACCAGGCCATGGCGAAGATCAGTCTTCAATGGATGAA
    ACGTGGAATTTTGATTATATTACGACGTTGTTAGACCGAATTTTAGATAAATATAAAGAT
    AAATCAATAACATTGTTTGGATATTCAATGGGTGGGCGTGTTGCATTATATTATGCAATT
    AATGGTCACATCCCTATATCTAATTTGATATTAGAAAGTACGTCACCAGGTATTAAAGAA
    GAAGCAAATCAATTGGAACGCCGTCTTGTTGATGATGCACGTGCTAAAGTATTAGACATA
    GCAGGTATTGAATTATTTGTTAATGATTGGGAAAAGTTGCCATTATTTCAATCGCAACTA
    GAATTACCAGTTGAAATACAACATCAAATAAGACAACAACGATTGTCTCAATCGCCACAT
    AAAATGGCCAAAGCATTAAGAGATTATGGTACAGGTCAAATGCCAAACTTATGGCCGCGC
    CTGAAAGAAATTAAAGTACCAACATTAATATTAGCTGGAGAATATGATGAAAAATTTGTA
    CAGATTGCGAAAAAAATGGCAAATTTAATTCCTAATAGTAAATGTAAATTAATTTCTGCT
    ACAGGTCATACAATTCATGTGGAAGATAGTGATGAATTTGATACAATGATATTAGGATTT
    TTAAAGGAGGAGCAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

⊟Protein[edit | edit source]

  • 2XMZ
    PDB
Protein Data Bank: 2XMZ

⊟General[edit | edit source]

  • locus tag: SACOL_RS05370 [old locus tag: SACOL1053 ]
  • symbol: SACOL_RS05370
  • description: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • length: 267
  • theoretical pI: 5.55003
  • theoretical MW: 30784.8
  • GRAVY: -0.409738

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.2.99.20?  ExPASy
    2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 300.5)
    and 10 more
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 83.3)
    proline-specific peptidase (TIGR01250; HMM-score: 78.7)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 61.6)
    pyrimidine utilization protein D (TIGR03611; HMM-score: 60.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 55.9)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 39.8)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 39.1)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 22.1)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 19.5)
    Plasmodium subtelomeric family (TIGR01607; HMM-score: 12.3)
  • TheSEED: see SACOL1053
  • ⊞PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 101.5)
    and 17 more
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 67.1)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 63.1)
    Ndr; Ndr family (PF03096; HMM-score: 23.7)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 23.1)
    PGAP1; PGAP1-like alpha/beta domain (PF07819; HMM-score: 19.9)
    Abhydrolase_11; Alpha/beta hydrolase domain (PF20408; HMM-score: 19.8)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 18.5)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 17.7)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 17.1)
    Esterase; Putative esterase (PF00756; HMM-score: 17)
    Mbeg1-like; Mbeg1-like (PF11187; HMM-score: 16.7)
    PepSY (CL0320) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 15.4)
    AB_hydrolase (CL0028) UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 15)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 14.3)
    DUF900; Alpha/beta hydrolase of unknown function (DUF900) (PF05990; HMM-score: 13.1)
    PETase; PET hydrolase-like (PF12740; HMM-score: 11.6)
    Peptidase_S15; X-Pro dipeptidyl-peptidase (S15 family) (PF02129; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9286
    • Cytoplasmic Membrane Score: 0.0512
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0189
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006007
    • TAT(Tat/SPI): 0.000299
    • LIPO(Sec/SPII): 0.001732
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 446072340 NCBI
  • RefSeq: WP_000150195 NCBI
  • UniProt: see SACOL1053

⊟Protein sequence[edit | edit source]

  • MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVITIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAINGHIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQLELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEKFVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL1053
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS05370&oldid=83021"
  • This page was last edited on 11 March 2016, at 03:41.
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