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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03175 [old locus tag: SACOL0614 ]
  • pan locus tag?: SAUPAN002344000
  • symbol: SACOL_RS03175
  • pan gene symbol?: bshB2
  • synonym:
  • product: bacillithiol biosynthesis deacetylase BshB2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03175 [old locus tag: SACOL0614 ]
  • symbol: SACOL_RS03175
  • product: bacillithiol biosynthesis deacetylase BshB2
  • replicon: chromosome
  • strand: -
  • coordinates: 648206..648871
  • length: 666
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (648206..648871) NCBI
  • BioCyc: SACOL_RS03175 BioCyc
  • MicrobesOnline: see SACOL0614

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACTGACGAAAGACATGTATTAGTAATATTTCCACATCCTGATGATGAAACCTTCTCA
    TCTGCAGGCACATTAGCAAATTATATCCAAAAAGGCATACCTGTAACATATGCATGTTTA
    ACCCTAGGACAAATGGGCAGAAATTTAGGTAATCCCCCATTTGCAACACGCGAATCATTA
    CCAAGTATTCGTGAGCGTGAACTAGAAGAAGCTTGTAAAGTTATCGGTATTACTGATTTA
    AGAAAAATGGGTTTACGTGACAAAACTGTCGAATTTGAACCCTATGAACATATAGATGGT
    ATGATTAAATCTTTAATCGATGATACAAATCCATCATTGATTATTTCATTTTATCCTGGA
    TATGCAGTACATCCTGATCACGAAGCAACAGCAGACGCTGTAATTCGAACGGTTGAACGC
    ATGCCTAAAGAAGAACGCCCTCGTTTAACTTTAGTTGCGTTCAGTAATGATGCAACCGAA
    GCTCTTGGAGAACCTGATATTCAAAATGATATTACAGATTTTAAAGAACTTAAAATTAAG
    GCATTTGAAGCACATGCTTCTCAAACAGGTCCATTTTTAAAACAATTAGCAAGTCCTGAA
    ATCGATGGTAAACAACACAGCTTTTTAACAGTCGAACCATATTGGACATATCACTTTAAA
    TCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    666

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03175 [old locus tag: SACOL0614 ]
  • symbol: SACOL_RS03175
  • description: bacillithiol biosynthesis deacetylase BshB2
  • length: 221
  • theoretical pI: 4.89444
  • theoretical MW: 24921
  • GRAVY: -0.415385

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis deacetylase BshB2 (TIGR04000; EC 3.-.-.-; HMM-score: 357.4)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis deacetylase BshB1 (TIGR04001; EC 3.-.-.-; HMM-score: 61.1)
    Cellular processes Cellular processes Detoxification N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (TIGR03445; EC 3.5.1.103; HMM-score: 44.8)
    Cellular processes Cellular processes Detoxification mycothiol conjugate amidase Mca (TIGR03446; HMM-score: 39.8)
  • TheSEED: see SACOL0614
  • PFAM:
    no clan defined PIG-L; GlcNAc-PI de-N-acetylase (PF02585; HMM-score: 107.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9963
    • Cytoplasmic Membrane Score: 0.0021
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0016
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013558
    • TAT(Tat/SPI): 0.004653
    • LIPO(Sec/SPII): 0.001036
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQMGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMIKSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATEALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYHFKS

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0614
  • protein localization: see SACOL0614
  • quantitative data / protein copy number per cell: see SACOL0614
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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