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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2157 [new locus tag: SACOL_RS11340 ]
  • pan locus tag?: SAUPAN005554000
  • symbol: SACOL2157
  • pan gene symbol?: lmrS
  • synonym:
  • product: EmrB/QacA family drug resistance transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2157 [new locus tag: SACOL_RS11340 ]
  • symbol: SACOL2157
  • product: EmrB/QacA family drug resistance transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2235375..2236817
  • length: 1443
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTAAAGTTGAATTAACAACCCGGAGACGAAACTTCATAGTTGCTGTTATGTTGATT
    AGTGCGTTTGTAGCTATTTTAAATCAAACATTATTAAATACAGCGTTACCTAGTATAATG
    AGAGAATTAAATATCAATGAAAGTACATCGCAATGGCTAGTTACTGGGTTTATGCTTGTT
    AATGGCGTCATGATACCTCTGACGGCATATCTAATGGATAGAATTAAAACTAGACCTTTA
    TACTTAGCGGCGATGGGGACATTTTTATTAGGTTCTATTGTTGCAGCCTTAGCTCCGAAT
    TTTGGAGTTTTAATGTTAGCTCGTGTAATTCAAGCGATGGGTGCAGGCGTACTTATGCCC
    TTAATGCAATTTACGTTATTTACATTGTTCAGTAAAGAACATCGAGGTTTTGCAATGGGA
    CTAGCAGGTTTAGTAATTCAATTTGCACCAGCAATAGGACCTACAGTTACAGGATTAATT
    ATTGATCAAGCGAGTTGGCGAGTTCCATTTATTATAATTGTAGGAATTGCTATACTTGCC
    TTTGTTTTCGGTTTGGTTTCAATCTCGAGTTACAATGAAGTGAAATATACGAAATTAGAT
    AAGCGTTCAGTAATGTATTCAACTATTGGGTTCGGGTTAATGCTATACGCATTTAGTAGT
    GCAGGAGATTTAGGATTTACAAGTCCAATAGTAATAGGTGCGTTGATATTAAGTATGGTT
    ATTATCTATTTATTTATACGTAGACAATTTAATATTACTAATGCACTTTTAAATTTAAGG
    GTTTTTAAAAATAGAACATTTGCATTATGTACGATTAGTTCAATGATTATAATGATGTCA
    ATGGTTGGACCTGCGCTGCTTATACCGCTATATGTTCAAAACAGTTTATCTTTATCTGCC
    TTGTTATCAGGACTTGTTATCATGCCTGGTGCAATAATAAATGGTATTATGTCAGTTTTT
    ACAGGTAAATTTTATGATAAGTATGGTCCTAGACCATTGATTTATACTGGTTTTACAATT
    TTAACAATTACTACAATTATGTTGTGTTTCTTGCATACAGACACATCTTATACGTATTTA
    ATAGTCGTATATGCAATTAGAATGTTTTCAGTTTCTTTACTCATGATGCCGATAAATACT
    ACAGGAATTAATTCTTTGAGAAATGAAGAAATCTCACATGGCACGGCTATTATGAACTTT
    GGTCGTGTAATGGCTGGTTCACTAGGCACAGCTTTAATGGTTACATTAATGAGTTTTGGT
    GCAAAAATATTTTTATCTACATCGCCATCGCATTTAACTGCAACTGAAATTAAACAGCAA
    TCCATTGCTATAGGGGTGGATATCTCATTTGCTTTTGTAGCTGTGCTTGTTATGGCAGCT
    TATGTGATAGCACTTTTTATAAGAGAACCTAAAGAAATAGAAAGTAATAGAAGGAAATTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1443

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2157 [new locus tag: SACOL_RS11340 ]
  • symbol: SACOL2157
  • description: EmrB/QacA family drug resistance transporter
  • length: 480
  • theoretical pI: 10.3424
  • theoretical MW: 52638
  • GRAVY: 0.849792

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 320.8)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 320.8)
    and 8 more
    multidrug resistance protein (TIGR00880; HMM-score: 69.6)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 52.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 40.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 34.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 32.7)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 22.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 17.3)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 12.4)
  • TheSEED  :
    • Drug resistance transporter, EmrB/QacA subfamily
  • ⊞PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 200.7)
    and 4 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 23.1)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 20.4)
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 19.1)
    no clan defined DUF5461; Family of unknown function (DUF5461) (PF17545; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • ⊞LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.166362
    • TAT(Tat/SPI): 0.133436
    • LIPO(Sec/SPII): 0.016231
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFGVLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLIIDQASWRVPFIIIVGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLIYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISHGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISFAFVAVLVMAAYVIALFIREPKEIESNRRKF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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