⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1571 [new locus tag: SA_RS08860 ]
pan locus tag^?: SAUPAN004428000
symbol: SA1571
pan gene symbol^?: dat
• synonym:
• product: D-alanine aminotransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1571 [new locus tag: SA_RS08860 ]
• symbol: SA1571
• product: D-alanine aminotransferase
• replicon: chromosome
• strand: -
• coordinates: 1801557..1802405
• length: 849
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124417 NCBI
• RefSeq: NP_374860 NCBI
• BioCyc: see SA_RS08860
• MicrobesOnline: 103886 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGAAAAAATTTTTTTAAATGGTGAGTTTGTAAGTCCAAGTGAAGCAAAGGTTTCATAC
  AACGACAGAGGATACGTATTTGGCGATGGTATTTATGAATACATTCGAGTATATAATGGT
  AAGTTATTTACAGTAACAGAACATTATGAAAGATTTTTACGTAGTGCCAATGAGATTGGT
  TTAGATTTAAATTATTCTGTAGAAGAATTAATTGAACTATCTCGTAAATTAGTTGATATG
  AATCAAATTGAAACTGGGGCAATTTATATTCAAGCAACGCGTGGTGTAGCTGAAAGGAAT
  CATAGCTTCCCGACACCTGAAGTAGAACCAGCAATTGTTGCTTATACAAAGAGTTATGAT
  CGTCCTTATGATCATTTAGAAAATGGTGTGAATGGTGTTACCGTTGAAGATATCCGATGG
  TTACGTTGCGACATTAAAAGCTTGAACTTATTAGGAAATGTATTAGCAAAAGAATATGCT
  GTGAAATATAATGCAGTTGAAGCAATTCAACATCGAGGTGAAACTGTAACTGAAGGATCT
  TCAAGTAATGCTTATGCAATTAAAGACGGTGTGATTTATACACATCCGATTAACAACTAT
  ATTCTTAATGGTATTACACGAATTGTAATTAAAAAAATTGCCGAAGACTATAACATCCCA
  TTTAAAGAAGAAACGTTTACTGTAGATTTCTTGAAAAACGCAGATGAAGTTATTGTTTCA
  AGTACTTCAGCTGAGGTTACACCTGTTATTAAATTAGATGGTGAACCAATTAATGATGGT
  AAAGTTGGCCCAATTACACGTCAACTACAAGAAGGATTTGAAAAGTATATAGAGTCACAC
  AGTATTTAA
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  849
  

⊟Protein

⊟General

• locus tag: SA1571 [new locus tag: SA_RS08860 ]
• symbol: SA1571
• description: D-alanine aminotransferase
• length: 282
• theoretical pI: 4.68119
• theoretical MW: 31907.6
• GRAVY: -0.316667

⊟Function

• ⊞⊞reaction:
  EC 2.6.1.21?  ExPASy
  D-amino-acid transaminase D-alanine + 2-oxoglutarate = pyruvate + D-glutamate
• ⊞⊞TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines D-amino-acid transaminase (TIGR01121; EC 2.6.1.21; HMM-score: 445.2)
  and 3 more
  Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01122; EC 2.6.1.42; HMM-score: 176.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate lyase (TIGR03461; EC 4.1.3.38; HMM-score: 91.5)
  Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01123; EC 2.6.1.42; HMM-score: 36.6)
• TheSEED  :
  • D-alanine aminotransferase (EC 2.6.1.21)
  Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-alanine aminotransferase (EC 2.6.1.21)
• ⊞⊞PFAM:
  no clan defined Aminotran_4; Amino-transferase class IV (PF01063; HMM-score: 147.1)
  and 1 more
  Sp-DndD; Small protein found in certain Dnd DNA modification systems (PF20306; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9237
  • Cytoplasmic Membrane Score: 0.0069
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0692
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009229
  • TAT(Tat/SPI): 0.000195
  • LIPO(Sec/SPII): 0.00093
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927327 NCBI
• RefSeq: NP_374860 NCBI
• UniProt: P99090 UniProt

⊟Protein sequence

• MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQEGFEKYIESHSI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• ⊟⊟interaction partners:

  SA1160	(nuc)	thermonuclease   (data from MRSA252)
  SA0865	(ppnK)	inorganic polyphosphate/ATP-NAD kinase   (data from MRSA252)
  SA0627		hypothetical protein   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1571 < SA1572

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)