PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1524 [new locus tag: SA_RS08580 ]
pan locus tag^?: SAUPAN004349000
symbol: SA1524
pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1524 [new locus tag: SA_RS08580 ]
• symbol: SA1524
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1739269..1740498
• length: 1230
• essential: yes DEG other strains

⊟Accession numbers

• Gene ID: 1124369 NCBI
• RefSeq: NP_374812 NCBI
• BioCyc: see SA_RS08580
• MicrobesOnline: 103838 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTCATTAAGAGATGAAGCATTGGAAATGCACAAACGTAATCAAGGTAAATTAGAAGTT
  AAACCAAATGTAAAAGTTACTAATAAAGAGGAATTAAGTTTAGCATACTCACCTGGCGTT
  GCTGAACCGTGTAAAGATATTTATGAAGATAAAAGAAAAGTATATGATTACACAATTAAA
  GGAAATACAGTTGCAGTTATTACTGATGGAACAGCGGTATTAGGTTTAGGTAACATTGGA
  CCTGAAGCAAGTATTCCTGTAATGGAAGGTAAAGCAGTATTATTCAAAAGCTTCGCTGGT
  ATCAATGGGGTGCCTATTGCGTTAAATACAACTGATACCGAAGAAATCATTAAAACAGTT
  AAGTTGTTAGAACCTAATTATGGTGGTATTAATTTAGAGGATATTTCGGCACCACGTTGT
  TTTGAAATTGAAGAACGATTGAAAAAAGAAACTAATATTCCGGTATTCCATGACGATCAA
  CATGGTACAGCAATTGTAACAATGGCAGGTTTGGTAAATGCATTGAGAGTTGTTAACAAA
  GATATTGCTAAAATAAAAGTTGTACTAAATGGTGCTGGTGCAGCAGGAATAGCCATTGTT
  AAATTACTATACGCGTATGGTGTAAGAAATATGGTTATGTGTGACTCAAGAGGCGCAATT
  TTTGAAGGACGTTCATATGGTATGAATCCTACGAAAGATGTTGTAGCTAAATGGACAAAT
  AAAGATAAGATTGAAGGGTCTTTAGAAGAAGTCGTAAAAGACGCAGATGTATTTATCGGA
  GTTTCTGTAGCTAATGCGCTGTCACAAGATATGGTTAAGAGTATGGCAGATAATCCAATT
  ATATTTGCAATGGCTAATCCAAATCCTGAAATAATACCTGATGATGCCAAAGCGGCAGGT
  GCACGAGTTGTTGGTACAGGACGTTCAGACTATCCTAACCAAATTAATAATGTATTAGCT
  TTCCCTGGTATTTTTAGAGGTGCATTAGAGGTTGAAGCTACACATATAAATGAAGAAATG
  AAAAAGGCAGCTGTAGAAGCGATTGCTGATTTAATCGATAGTTCTGAATTAAATGAAGAC
  TACTGTATCCCAGGACCGTTTGATAAACGTGTAGCGCCATCAGTTGCTCGTAATGTTGCT
  AAAGCGGCAATGGAATCTGGAGTAGCTAGGATTGAAGTTGATCCGCAAGATGTGTATGAT
  AAAACAATGAAACTTACAGATTTACAATAA
  60
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  1230
  

⊟Protein

⊟General

• locus tag: SA1524 [new locus tag: SA_RS08580 ]
• symbol: SA1524
• description: hypothetical protein
• length: 409
• theoretical pI: 4.81628
• theoretical MW: 44251.5
• GRAVY: -0.127384

⊟Function

• TIGRFAM:
  E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 13.3)
• TheSEED  :
  • Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (EC 1.1.1.40)
  Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  NADP-dependent malic enzyme (EC 1.1.1.40)
• ⊞PFAM:
  AA_dh_N (CL0603) malic; Malic enzyme, N-terminal domain (PF00390; HMM-score: 111.1)
  NADP_Rossmann (CL0063) Malic_M; Malic enzyme, NAD binding domain (PF03949; HMM-score: 90.9)
  and 5 more
  ThiF; ThiF family (PF00899; HMM-score: 17.1)
  ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 16.6)
  no clan defined Gly_rich_SFCGS; Glycine-rich SFCGS (PF14272; HMM-score: 13.4)
  NADP_Rossmann (CL0063) FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.6)
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, Mn²⁺
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7877
  • Cytoplasmic Membrane Score: 0.0155
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.1963
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012323
  • TAT(Tat/SPI): 0.003306
  • LIPO(Sec/SPII): 0.001611
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927279 NCBI
• RefSeq: NP_374812 NCBI
• UniProt: A0A0H3JQE7 UniProt

⊟Protein sequence

• MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPCKDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGINGVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQHGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRGAIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMADNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEATHINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIEVDPQDVYDKTMKLTDLQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications