⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA0584 [new locus tag: SA_RS03365 ]
pan locus tag^?: SAUPAN002501000
symbol: SA0584
pan gene symbol^?: mnhG2
• synonym:
• product: monovalent cation/H+ antiporter subunit G

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0584 [new locus tag: SA_RS03365 ]
• symbol: SA0584
• product: monovalent cation/H+ antiporter subunit G
• replicon: chromosome
• strand: +
• coordinates: 675373..675810
• length: 438
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123390 NCBI
• RefSeq: NP_373838 NCBI
• BioCyc: see SA_RS03365
• MicrobesOnline: 102864 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGAAATAACAAAAGAAATCTTTAGTCTTATTGCTGCTGTGATGTTGTTGTTAGGTAGT
  TTTATTGCTCTTATTAGTGCAATAGGTATCGTGAAATTCCAAGATGTTTTCTTAAGAAGT
  CACGCTGCGACAAAAAGTTCAACTTTATCCGTGTTATTAACTTTAATCGGTGTATTAATT
  TATTTTATTGTGAATACAGGATTTTTCAGTGTGCGTTTATTACTGTCACTTGTTTTTATT
  AATTTAACTTCACCAGTCGGCATGCACTTAGTCGCTCGCGCTGCTTATCGCAACGGCGCT
  TATATGTATCGAAAAAATGATGCTCACACACATGCATCAATATTATTAAGTTCAAATGAA
  CAAAACTCTACAGAAGCATTACAATTACGTGCTAAAAAACGAGAAGAGCATCGTAAGAAA
  TGGTATCAAAACGATTGA
  60
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  438
  

⊟Protein

⊟General

• locus tag: SA0584 [new locus tag: SA_RS03365 ]
• symbol: SA0584
• description: monovalent cation/H+ antiporter subunit G
• length: 145
• theoretical pI: 10.4527
• theoretical MW: 16302
• GRAVY: 0.342069

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation/proton antiporter, MnhG/PhaG subunit (TIGR01300; HMM-score: 90)
  and 1 more
  exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 15.8)
• TheSEED  :
  • Na(+) H(+) antiporter subunit G (TC 2.A.63.1.3)
  Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit G (TC 2.A.63.1.3)
• ⊞⊞PFAM:
  no clan defined PhaG_MnhG_YufB; Na+/H+ antiporter subunit (PF03334; HMM-score: 79)
  and 3 more
  DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 12.2)
  DUF7472; Family of unknown function (DUF7472) (PF24284; HMM-score: 8.3)
  TSPAN_4TM-like (CL0347) Tetraspanin; Tetraspanin family (PF00335; HMM-score: 6.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• ⊞⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9996
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0004
• ⊞⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008271
  • TAT(Tat/SPI): 0.0003
  • LIPO(Sec/SPII): 0.021697
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 15926305 NCBI
• RefSeq: NP_373838 NCBI
• UniProt: Q7A722 UniProt

⊟Protein sequence

• MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAATKSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYMYRKNDAHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0577 > SA0578 > SA0579 > SA0580 > SA0581 > SA0582 > SA0583 > SA0584

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications