PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_2224 [new locus tag: NWMN_RS12825 ]
pan locus tag^?: SAUPAN005803000
symbol: NWMN_2224
pan gene symbol^?: glvC
• synonym:
• product: PTS system, alpha-glucoside-specific IIBC component

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2224 [new locus tag: NWMN_RS12825 ]
• symbol: NWMN_2224
• product: PTS system, alpha-glucoside-specific IIBC component
• replicon: chromosome
• strand: -
• coordinates: 2444469..2446073
• length: 1605
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331348 NCBI
• RefSeq: YP_001333258 NCBI
• BioCyc:
• MicrobesOnline: 3707826 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATGCGATAAAGCGTTTCGGTAGTGCAATGATTGTACCGGTATTAATGTTCGCTTTC
  TTTGGGATAGTGCTTGGCTTTGCGACATTATTTAAAAATCCTACAATCATGGGGAGTTTA
  GCAGATCAACATACGTTTTGGTTTAAATTTTGGTCAGTAATAGAATCTGGTGGCTGGGTT
  ATTTTTACGCATATGGAAGTTGTATTTGTTGTAGGTCTACCACTTTCTTTAGCTAAAAAA
  GCACCAGGTCATGCAGCACTTGCAGCATTGATGGGTTACTTAATGTTTAATACATTTATA
  AATGCCATTTTAACACAATGGCCACATACGTTTGGCGCTAATTTAGAAAAAGGCGTTGAA
  AATGTACCTGGTTTAAAATCAATTGCAGGTATTGCCACATTGGATACGAATATTCTAGGT
  GGTATTATTATTTCAGCTATTATTACTTGGATTCACAATAGATACTATAGTAAACGATTA
  CCAGAAATGGTTGGTGTATTCCAAGGTTTAACATTTGTAGTTACGATTTCATTTTTTGTG
  ATGTTACCATTAGCAGCAATTACTTGTGTTATTTGGCCAACTGTTCAGCACGGTATTGGT
  TCAATGCAACACTTTATCATTGCTTCAGGCTATATCGGTGTATGGTTGTATCACTTCTTA
  GAACGTGTATTAATACCAACAGGATTACATCACTTTATTTATGCACCAATTGAAGTTGGA
  CCAGTAGTAGTCAATCACGGCTTGAAAGCTGAATGGTTACAACATTTAAATGAGTTTGCT
  AAGAGTACTAAGCCATTAAAAGAGCAATTCCCTTATGGCTTTATGCTTCAAGGAAATGGG
  AAAGTATTTGGATGTTTAGGTATTGCTTTAGCAATGTATGCTACGACACCTAAAGAAAAT
  CGTAAAAAGGTGGCGGCACTACTTATTCCTGCCACATTAACAGCAGTCGTTGTAGGTATT
  ACTGAGCCATTAGAGTTTACATTCTTATTCATCGCACCATACTTATTTGTTTTACACGCA
  GTTTTAGCAGCTTCAATGGATACTTTAATGTACGCATTTGGTGTTGTAGGTAACATGGGT
  GGTGGCTTGTTAGACTTTATTTCAACAAACTGGTTGCCATTAGGTAAAGAACATTGGGGT
  ACTTATGTTGCACAAGTTATTATCGGTCTGATATTTGTAGCGATTTACTTCTTCTTATTC
  AGATTCTTAATTTTGAAATTTGATATTCCATTACCTGGACGTAAGAAAACAGAAGAGGAA
  GTTAAATTATTTTCTAAGCAAGATTACAAAAATAAAAAAGGTGATAGCGTAGATTCTAAA
  CGTGCTTCATCTGGTAATGAATACGAAGACAAAGCTGCATATTACCTCGACGGTTTAGGC
  GGCAAAGAAAATATCAAAGATGTTACTAACTGTACAACAAGATTACGCTTAACTGTTTAT
  GATGAAAGTAAAGTTGCAGATACGGAATATTTCACGCATCAACAAATGGCACACGGATTA
  GTAAAGAGTGGTAAGAGTATCCAAGTTGTAGTAGGTATGACAGTACCACAAGTTCGTGAA
  GCATTTGAACAAATGGTTGAGGATCAATCGCCCGAAGATAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_2224 [new locus tag: NWMN_RS12825 ]
• symbol: NWMN_2224
• description: PTS system, alpha-glucoside-specific IIBC component
• length: 534
• theoretical pI: 8.64496
• theoretical MW: 59339.2
• GRAVY: 0.364607

⊟Function

• ⊞TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 966.1)
  and 11 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 290.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 278.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 277.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 237.7)
  Signal transduction Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 237.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 161.9)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 58.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 56)
  Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 56)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 52.8)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 50.2)
• TheSEED  :
  • PTS system, maltose-specific IIB component (EC 2.7.1.69)
  • PTS system, maltose-specific IIC component
• ⊞PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 212.2)
  and 2 more
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 54.7)
  Ldr_toxin; Toxin Ldr, type I toxin-antitoxin system (PF13940; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• ⊞DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9998
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0002
• ⊞LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.065349
  • TAT(Tat/SPI): 0.00115
  • LIPO(Sec/SPII): 0.019006
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222436 NCBI
• RefSeq: YP_001333258 NCBI
• UniProt: A0A0H3KEP1 UniProt

⊟Protein sequence

• MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQHTFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINAILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRLPEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYHFLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFMLQGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPYLFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIFVAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYEDKAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQVVVGMTVPQVREAFEQMVEDQSPEDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• ⊞regulators: GlvR* (activation) regulon, CcpA regulon

  GlvR*	(TF)	important in Maltose utilization; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications