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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

organism: Staphylococcus aureus N315

locus tag: SA0681 [new locus tag: SA_RS03880 ]

pan locus tag^?: SAUPAN002621000

symbol: SA0681

pan gene symbol^?: bmrU

synonym:
product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SA0681 [new locus tag: SA_RS03880 ]
symbol: SA0681
product: hypothetical protein
replicon: chromosome
strand: -
coordinates: 777890..778807
length: 918
essential: no DEG other strains

⊟Accession numbers[edit | edit source]

Gene ID: 1123488 NCBI
RefSeq: NP_373936 NCBI
BioCyc: see SA_RS03880
MicrobesOnline: 102962 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAAAATAAATATACACATGGTGTCCTCTTTTACCATGAACACAGTGGATTAAAAAAT
ATTAATCAAGGTATTGGGGAAGTTACAACAGCATTAAGTTCAATTTGTAAGCATCTTTCT
ATTCAATTGAGTGAAAACGAAGGTGACATTATTAAATATTGCCAAGAAATCAAAACTAAA
AACTATGCAAAAGATGTAGATATATTATTTATTCTTGGCGGTGATGGCACGGTTAACGAA
CTTATAAATGGTGTTATGTCACATGACCTTCAACTCCCTATTGGCATTTTACCAGGCGGT
ACTTTTAATGATTTTACAAAAACGCTAAATATAGCACCTAATCATAAACAAGCTAGTGAA
CAAATGATTTCTGCACAAGTTGGTACATACGATGTAATTAAAATAAATAATCAATATGCA
CTCAACTTTGTTGGCTTAGGTCTCATTGTTCAAAACGCTGAAAACGTACAAGATGGTTCA
AAAGATATATTTGGTAAATTGAGTTATATTGGATCAACTGTCAAAACACTGCTAAATCCA
ACACAATTTAATTATCAATTATCAATAGATGATAAGACATATTCTGGCGAAACGACCATG
ATACTAACAGCAAATGGTCCATTCATTGGTGGTAGTCGTATACCATTGACTGATTTGTCT
CCTCAAGATGGTGAACTGAACACTTTCATTTTTAATGAACAAAGTTTCAGTATTTTAAAT
GATATTTTTAAAAAGCGTGATAGCATGAATTGGAATGAAATTACTCAAGGTATTGAGCAT
ATACCTGGTAAAAAGATTTCACTTACAACAGATCCAGCAATGAAAGTTGATATTGATGGC
GAAATTTCATTAGAAACACCTATTGATATTGAGGTTATACCAAATGCTATTCAGTTACTT
ACTGTCAATGATTTGTAA
60
120
180
240
300
360
420
480
540
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840
900
918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SA0681 [new locus tag: SA_RS03880 ]
symbol: SA0681
description: hypothetical protein
length: 305
theoretical pI: 4.49354
theoretical MW: 33611.8
GRAVY: -0.171148

⊟Function[edit | edit source]

reaction:
EC 2.7.1.-? ExPASy
⊞TIGRFAM:
Unknown function Enzymes of unknown specificity lipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 169.1)
and 1 more
Unknown function Enzymes of unknown specificity lipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 77.1)
TheSEED :
- Transcription regulator [contains diacylglycerol kinase catalytic domain]
⊞PFAM:
PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 98.4)
and 2 more
NAD_kinase_C (CL0701) YegS_C; YegS C-terminal NAD kinase beta sandwich-like domain (PF19279; HMM-score: 48.3)
PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Mg²⁺
effectors:

⊟Localization[edit | edit source]

⊞
PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
⊞
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.848
- Cytoplasmic Membrane Score: 0.1474
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0044
⊞
LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
⊞
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.016346
- TAT(Tat/SPI): 0.00054
- LIPO(Sec/SPII): 0.002097
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 15926403 NCBI
RefSeq: NP_373936 NCBI
UniProt: Q7A6T6 UniProt

⊟Protein sequence[edit | edit source]

MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLSENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTFNDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGSKDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTDLSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKVDIDGEISLETPIDIEVIPNAIQLLTVNDL

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

data available for COL, NCTC8325, Newman

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: no data available

⊟Protein stability[edit | edit source]

half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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