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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0523 [new locus tag: SACOL_RS02670 ]
  • pan locus tag?: SAUPAN002215000
  • symbol: SACOL0523
  • pan gene symbol?: —
  • synonym:
  • product: Orn/Lys/Arg decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0523 [new locus tag: SACOL_RS02670 ]
  • symbol: SACOL0523
  • product: Orn/Lys/Arg decarboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 535057..536394
  • length: 1338
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGCAACCTATTTTAAATAAATTAGAAAGTTTAAATCAAGAAGAAGCGATTTCTTTG
    CATGTTCCGGGTCATAAAAATATGACTATCGGTCATTTATCTCAATTATCAATGACAATG
    GATAAAACTGAAATACCTGGATTAGATGATTTACATCATCCTGAAGAAGTCATTTTGGAA
    AGTATGAAGCAGGTGGAGAAACATTCAGATTATGATGCTTATTTCTTAGTGAATGGCACC
    ACTTCAGGAATATTATCTGTCATCCAGTCTTTTTCACAGAAAAAAGGCGATATCTTAATG
    GCAAGAAATGTACATAAATCTGTGTTACATGCGCTCGATATTAGCCAACAAGAAGGGCAT
    TTTATTGAAACGCATCAAAGTCCGTTAACGAATCATTATAATAAAGTTAATTTAAGCCGT
    TTGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACA
    TTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGAC
    GAAGCACACGGCGCGCACTTTGGATTGCAAGGATTTCCAGATTCTACATTAAATTATCAA
    GCTGACTATGTTGTTCAATCTTTTCATAAAACGTTACCAGCTTTAACGATGGGCTCGGTA
    CTTTATATTCATAAAAATGCACCTTATAGAGAAACTATTATAGAATATCTAAGCTACTTC
    CAAACATCTAGTCCTTCGTATTTGATTATGGCTAGTTTAGAGTCAGCTGCCGAGTTCTAT
    AAAACATATGATAGTACCGTGTTTTTTGATAAGAGAGCGCAATTAATCGAATGTTTGGAG
    AAGAAGGGTTTTGAAATGCTTCAAGTTGATGATCCGTTGAAGTTGCTGATAAAATATGAA
    GGTTTTACAGGTCATGATATTCAAAATTGGTTTATGAATGCACATATCTATTTAGAATTA
    GCGGACGACTATCAAGCATTAGCGATATTGCCGTTATGGCATCATGATGATACGTATTTA
    TTTGATTCGCTTTTACGTAAAATTGAAGATATGATTTTACCGAAAAAATCAGTTTCTAAA
    GTTAAACAAACACAACTTTTAACAACTGAAGGTAACTATAAACCAAAACGCTTTGAATAT
    GTTACTTGGTGTGATTTGAAAAAGGCAAAAGGTAAAGTTCTGGCGCGACATATTGTCCCG
    TATCCGCCAGGGATTCCTATTATTTTCAAAGGAGAAACAATAACTGAAAATATGATAGAA
    TTGGTAAATGAATATCTGGAAACTGGAATGATAGTTGAAGGAATTAAAAATAATAAAATT
    TTAGTTGAGGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0523 [new locus tag: SACOL_RS02670 ]
  • symbol: SACOL0523
  • description: Orn/Lys/Arg decarboxylase
  • length: 445
  • theoretical pI: 6.02953
  • theoretical MW: 51021
  • GRAVY: -0.29618

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    ornithine decarboxylase SpeF (TIGR04301; EC 4.1.1.17; HMM-score: 29.9)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 29)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 26.5)
    putative ornithine decarboxylase (TIGR04318; EC 4.1.1.-; HMM-score: 25.9)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 25.1)
    and 2 more
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 15.3)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 11.4)
  • ⊞TheSEED  :
    • Arginine decarboxylase (EC 4.1.1.19)
    • Lysine decarboxylase (EC 4.1.1.18)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Arginine decarboxylase (EC 4.1.1.19)
    and 2 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Arginine decarboxylase (EC 4.1.1.19)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Lysine degradation  Lysine decarboxylase (EC 4.1.1.18)
  • ⊞PFAM:
    PLP_aminotran (CL0061) OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 149.5)
    and 3 more
    no clan defined OKR_DC_1_C; Orn/Lys/Arg decarboxylase, C-terminal domain (PF03711; HMM-score: 34.9)
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 27.6)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 19.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9709
    • Cytoplasmic Membrane Score: 0.0241
    • Cell wall & surface Score: 0.0011
    • Extracellular Score: 0.0039
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003136
    • TAT(Tat/SPI): 0.000237
    • LIPO(Sec/SPII): 0.000308
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTEIPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMARNVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGETFNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMGSVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLIECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWHHDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGKVLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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