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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03290 [old locus tag: SACOL0637 ]
  • pan locus tag?: SAUPAN002372000
  • symbol: SACOL_RS03290
  • pan gene symbol?: mvaD
  • synonym:
  • product: diphosphomevalonate decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03290 [old locus tag: SACOL0637 ]
  • symbol: SACOL_RS03290
  • product: diphosphomevalonate decarboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 669084..670067
  • length: 984
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (669084..670067) NCBI
  • BioCyc: SACOL_RS03290 BioCyc
  • MicrobesOnline: see SACOL0637

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGATTAAAAGTGGCAAAGCACGTGCACATACGAATATTGCACTTATAAAATATTGGGGT
    AAAAAAGATGAAGCACTAATCATTCCAATGAATAATAGCATATCTGTTACATTAGAAAAA
    TTTTACACTGAAACGAAAGTCACTTTTAACGACCAGTTAACACAGGATCAATTTTGGTTG
    AATGGTGAAAAGGTTAGTGGCAAAGAATTAGAGAAAATTTCAAAATATATGGATATTGTC
    AGAAATAGAGCTGGCATCGATTGGTATGCAGAAATTGAAAGCGACAATTTTGTACCAACA
    GCAGCAGGGTTGGCTTCATCGGCAAGCGCATATGCAGCTTTAGCAGCAGCTTGTAATCAA
    GCGCTAGACATGCAGCTGTCAGATAAGGATTTATCGAGATTGGCGCGAATTGGTTCGGGT
    TCTGCGTCGCGTAGTATTTATGGTGGATTTGCAGAATGGGAAAAAGGGTATAGTGATGAG
    ACGTCATATGCCGTTCCACTTGAATCGAATCATTTTGAAGATGACCTTGCCATGATATTT
    GTTGTGATTAATCAACATTCTAAAAAGGTACCTAGTCGATATGGTATGTCATTGACACGA
    AACACATCAAGGTTTTATCAATATTGGTTAGATCATATTGATGAAGATTTAGCTGAAGCA
    AAAGCAGCGATTCAAGACAAAGATTTTAAACGCCTTGGTGAAGTAATTGAAGAAAATGGT
    TTGCGTATGCATGCCACGAATCTAGGATCAACACCGCCGTTCACATATCTTGTGCAAGAA
    AGTTATGATGTCATGGCGCTTGTTCACGAATGCCGAGAAGCGGGGTATCCGTGTTATTTT
    ACAATGGATGCGGGACCTAATGTGAAAATACTTGTAGAAAAGAAAAACAAGCAACAGATT
    ATAGATAAATTATTAACACAGTTTGATAATAACCAAATTATTGATAGTGACATTATTGCC
    ACAGGAATTGAAATAATTGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03290 [old locus tag: SACOL0637 ]
  • symbol: SACOL_RS03290
  • description: diphosphomevalonate decarboxylase
  • length: 327
  • theoretical pI: 4.83909
  • theoretical MW: 36822.3
  • GRAVY: -0.34893

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.1.1.33?  ExPASy
    Diphosphomevalonate decarboxylase ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO2
  • ⊞TIGRFAM:
    Metabolism Central intermediary metabolism Other diphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 398.5)
    and 5 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 45.1)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 25.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 16.9)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 16.2)
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 16)
  • TheSEED: see SACOL0637
  • ⊞PFAM:
    S5 (CL0329) MVD-like_N; Diphosphomevalonate decarboxylase-like N-terminal domain (PF22700; HMM-score: 228.9)
    and 2 more
    GHMP_C (CL0677) MDD_C; Mevalonate 5-diphosphate decarboxylase C-terminal domain (PF18376; HMM-score: 91.3)
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 38.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9723
    • Cytoplasmic Membrane Score: 0.0222
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0054
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004285
    • TAT(Tat/SPI): 0.000159
    • LIPO(Sec/SPII): 0.000933
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTETKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAAGLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDETSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLAEAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGYPCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0637
  • protein localization: see SACOL0637
  • quantitative data / protein copy number per cell: see SACOL0637
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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