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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07710 [old locus tag: NWMN_1369 ]
  • pan locus tag?: SAUPAN003918000
  • symbol: NWMN_RS07710
  • pan gene symbol?: bshA
  • synonym:
  • product: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07710 [old locus tag: NWMN_1369 ]
  • symbol: NWMN_RS07710
  • product: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA
  • replicon: chromosome
  • strand: -
  • coordinates: 1535651..1536793
  • length: 1143
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1535651..1536793) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1369

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGATAGGTATAACATGTTACCCGTCCATGGGTGGTTCTGGAATTATTGCCACAGAA
    TTAGGAATTAAATTAGCAGAGCGAGGTCACGAAGTACATTTTATCACTTCAAATATCCCG
    TTTAGAATAAGAAAACCATTACCGAATATGATTTTTCATCAAGTTGAAGTGAATCAATAT
    GCAGTATTTCAGTATCCACCGTATGATATTACGTTAAGTACTAAAATCGCTGAAGTTATT
    AAAGAATATGATCTTGATTTGTTGCATATGCACTATGCTGTACCTCATGCAATTTGTGGG
    ATTTTAGCTCGTGAGATGTCAGGTAAAGATATAAAAATTATGACAACGCTACACGGTACT
    GATATTACTGTTTTGGGCTATGATCATTCACTCCAAGGTGCAATTAAATTTGGCATTGAA
    AAAAGTGATATTGTGACAAGTGTGAGTAAATCTTTAGCACAAGAAACACATGAAATTATT
    GAAACTAACAAAGAAATTATACCGATTTATAATTTTGTACGTGAAAATGAGTTTCCAACT
    AAACATAACACAGCATTAAAATCACAATTTGGTATAGCACCAGATGAAAAAGTGTTAATA
    CATGTATCGAATTTTAGACAAGTAAAACGCATAGATACTATTATAGAAACATTTGCAAAA
    GTACGTGAAAAGATACCTAGTAAATTAATTTTACTTGGAGATGGTCCTGAATTAGTGCCA
    ATGCGTCAATTGACGAAAGAGTTAAACGTAGAAGAGGATGTTTTATTTTTAGGGAAACAA
    GATTGCGTAAGTGAATTTTACCAATTATCTGACTTAGTATTATTATTAAGTGAAAAAGAA
    AGCTTTGGACTAACTTTACTTGAGGCTATGAAAACAGGCGTCGTTCCAATTGGTTCAAAT
    GCCGGTGGAATTAAAGAGGTTATTAAACATGGTGAAACTGGATTTGTCGTAGATGTGGGT
    GATTGTGATTCAGCGAGTGACTATGCTATCCGATTACTTGAAGATAAGGTGTTATACAAC
    AAACTTCAGAAAAACATGCTAGCAGATATTGCTGAACGTTTTGGATCAGAACTTATTACA
    GATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAAAAGTAAAGGCGAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07710 [old locus tag: NWMN_1369 ]
  • symbol: NWMN_RS07710
  • description: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA
  • length: 380
  • theoretical pI: 5.75583
  • theoretical MW: 42795.1
  • GRAVY: -0.127632

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 553.2)
    and 13 more
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 93.2)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 78.9)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 75.7)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 61.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 61.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 54.8)
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 41.9)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 38.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 30.1)
    colanic acid biosynthesis glycosyltransferase WcaI (TIGR04007; EC 2.4.-.-; HMM-score: 29.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 26.9)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 25.3)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 24)
  • TheSEED: see NWMN_1369
  • ⊞PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 132.5)
    and 7 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 99.9)
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 75.3)
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 49.6)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 32)
    Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 22.9)
    GT4-conflict; Family 4 Glycosyltransferase in conflict systems (PF20706; HMM-score: 18.3)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7861
    • Cytoplasmic Membrane Score: 0.1701
    • Cell wall & surface Score: 0.0027
    • Extracellular Score: 0.0411
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018066
    • TAT(Tat/SPI): 0.000537
    • LIPO(Sec/SPII): 0.004663
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIRKPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGILAREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEIIETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETFAKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLLSEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLEDKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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