PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS08515 [old locus tag: SA1512 ]
pan locus tag^?: SAUPAN004312000
symbol: SA_RS08515
pan gene symbol^?: mutM
• synonym:
• product: DNA-formamidopyrimidine glycosylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08515 [old locus tag: SA1512 ]
• symbol: SA_RS08515
• product: DNA-formamidopyrimidine glycosylase
• replicon: chromosome
• strand: -
• coordinates: 1721209..1722081
• length: 873
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1721209..1722081) NCBI
• BioCyc: SA_RS08515 BioCyc
• MicrobesOnline: see SA1512

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCCCGAATTACCAGAAGTAGAACATGTAAAAAGAGGAATTGAACCCTACGTTATAAAT
  CAAAAAATTGAACACGTTATATTTTCGGATAAAGTAATCGAAGGTAAAGCTCAAGGCAAG
  GAAACAATTATAAAAGGAATTGAATTAGATACTTTTAAAACTTTATCGGAAGGCTATACC
  ATTACAAATGTAGAACGAAGAAGTAAATATATCGTTTTTCAGCTAGATAATAAACGAGAA
  CAACGTACATTAATTAGTCATTTAGGTATGGCGGGTGGTTTTTTCATTGTAGATGAACTT
  GAAGATATTATGATACCTAATTATCGTAAGCATTGGCATGTTATTTTTGAGTTGTCAAAT
  GACAAAAAGTTAATTTATTCAGATATACGTCGTTTTGGAGAAATTAGAAATGTTGCGTCT
  GTAGCATCTTATCCTTCATTTTTAGAAATAGCGCCTGAACCTTTTACAAATGAAGCATTG
  ACGTATTATTTAAATCGAATTCATCAACAGTCTAATAAGAATAAACCAATCAAACAAGTG
  ATTTTGGATCATAAAGTAATCGCAGGTTGCGGAAATATTTACGCATGTGAAGCGTTGTTT
  CGTGCAGGTGTTTTACCAGATAAAAAAGTAAAAGACTTAACACACCAACAACAAGAAATG
  GTTTTTTATTATGTTAGAGAAGTACTAGAAGAAGGTATTAAGCATGGCGGTACAAGTATT
  TCAGATTATCGTCATGCTGATGGAAAAACTGGTGAGATGCAATTGCATCTTAATGTATAT
  AAGCAGCCGGTATGCAAAGTGTGTGGAAGTCAAATTGAAACAAAAATCATCGCGACAAGA
  AATAGTCATTATTGTCCAGTATGTCAAAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS08515 [old locus tag: SA1512 ]
• symbol: SA_RS08515
• description: DNA-formamidopyrimidine glycosylase
• length: 290
• theoretical pI: 8.1364
• theoretical MW: 33399.2
• GRAVY: -0.396897

⊟Function

• ⊞reaction:
  EC 3.2.2.23?  ExPASy
  DNA-formamidopyrimidine glycosylase Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
  EC 4.2.99.18?  ExPASy
  DNA-(apurinic or apyrimidinic site) lyase The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
• ⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA-formamidopyrimidine glycosylase (TIGR00577; EC 3.2.2.23; HMM-score: 234.4)
  and 1 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS13 (TIGR03631; HMM-score: 15.2)
• TheSEED: see SA1512
• ⊞PFAM:
  no clan defined Fapy_DNA_glyco; Formamidopyrimidine-DNA glycosylase N-terminal domain (PF01149; HMM-score: 102.7)
  and 7 more
  H2TH (CL0303) H2TH; Formamidopyrimidine-DNA glycosylase H2TH domain (PF06831; HMM-score: 73.8)
  Tbcl_zf (CL0839) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 24.4)
  H2TH (CL0303) NFACT_N; NFACT N-terminal and middle domains (PF05833; HMM-score: 17.3)
  HTH (CL0123) HTH_66; Putative DNA-binding HTH domain (PF21321; HMM-score: 16.8)
  Zn_Beta_Ribbon (CL0167) DZR_2; Double zinc ribbon domain (PF18912; HMM-score: 12.7)
  CDA (CL0109) Deam_C; Putative cytidine deaminase, C-terminal domain (PF24241; HMM-score: 12.4)
  HTH (CL0123) Cdh1_DBD_1; Chromodomain helicase DNA-binding domain 1 (PF18196; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9951
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0041
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005618
  • TAT(Tat/SPI): 0.000165
  • LIPO(Sec/SPII): 0.000521
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447037198 NCBI
• RefSeq: WP_001114454 NCBI
• UniProt: see SA1512

⊟Protein sequence

• MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETIIKGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELEDIMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEALTYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQEMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKIIATRNSHYCPVCQK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications