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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • pan locus tag?: SAUPAN002473000
  • symbol: NWMN_RS03315
  • pan gene symbol?: argS
  • synonym:
  • product: arginine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • product: arginine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 659531..661192
  • length: 1662
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (659531..661192) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0579

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATATTATTGATCAAGTGAAACAAACATTAGTAGAAGAAATTGCAGCAAGTATTAAC
    AAAGCAGGATTAGCAGATGAGATTCCTGATATTAAAATTGAAGTTCCTAAAGATACAAAA
    AATGGAGATTATGCTACTAATATTGCGATGGTACTGACTAAGATTGCAAAGCGTAATCCT
    CGTGAAATTGCTCAAGCGATTGTTGATAACTTAGATACTGAAAAAGCACATGTAAAACAA
    ATTGACATTGCTGGTCCAGGATTCATTAATTTTTACTTAGATAATCAGTATTTAACAGCA
    ATTATTCCTGAAGCAATTGAAAAAGGTGATCAATTTGGACATGTAAATGAATCAAAAGGT
    CAAAATGTATTGCTTGAGTATGTTTCAGCTAACCCTACAGGAGATTTACATATTGGTCAT
    GCTAGAAATGCAGCAGTTGGTGATGCTTTAGCTAATATTTTAACTGCAGCTGGCTATAAT
    GTAACACGTGAATATTATATTAATGATGCTGGTAATCAAATTACTAACTTAGCGCGTTCG
    ATTGAAACACGTTTCTTTGAAGCTTTAGGTGACAATAGTTATTCAATGCCAGAAGATGGC
    TATAATGGAAAAGATATTATTGAAATAGGTAAAGATTTAGCAGAGAAACACCCTGAAATT
    AAAGATTATTCTGAAGAAGCACGTTTGAAAGAATTTAGAAAATTAGGCGTAGAATACGAA
    ATGGCTAAATTGAAAAATGATTTAGCAGAGTTCAATACGCATTTTGATAATTGGTTTAGT
    GAAACATCTTTATATGAAAAAGGAGAAATTCTTGAAGTTTTAGCAAAAATGAAAGAATTA
    GGTTATACGTATGAAGCTGATGGCGCTACATGGTTACGTACAACTGATTTTAAAGACGAC
    AAAGACAGAGTATTAATTAAAAATGACGGTACATATACGTATTTCTTACCAGATATTGCG
    TACCACTTCGATAAAGTAAAACGTGGTAATGACATTTTAATCGATTTATTTGGTGCTGAT
    CATCATGGTTATATTAATCGTTTGAAAGCATCTCTTGAAACGTTTGGTGTAGATAGTAAT
    CGTTTAGAAATTCAAATCATGCAAATGGTTCGTTTAATGGAAAATGGTAAAGAAGTGAAG
    ATGAGTAAACGTACTGGTAATGCGATTACATTAAGAGAAATTATGGACGAAGTTGGCGTT
    GACGCTGCACGTTATTTCTTAACTATGCGTAGTCCTGATAGTCACTTTGATTTTGATATG
    GAATTAGCGAAAGAGCAATCTCAAGACAATCCAGTTTACTATGCTCAATATGCACATGCG
    CGTATTTGTTCAATTTTAAAACAAGCGAAAGAGCAAGGTATTGAAGTGACTGCTGCGAAT
    GATTTTACAACGATTACTAATGAAAAAGCGATTGAATTGTTGAAAAAAGTAGCTGATTTC
    GAACCTACAATTGAAAGTGCTGCTGAGCATAGATCGGCACATAGAATTACTAATTATATT
    CAAGATTTAGCTTCTCATTTCCATAAATTCTATAATGCTGAAAAAGTGTTAACAGATGAT
    ATTGAAAAAACAAAAGCACATGTTGCTATGATTGAAGCGGTCAGAATTACATTGAAAAAT
    GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS03315 [old locus tag: NWMN_0579 ]
  • symbol: NWMN_RS03315
  • description: arginine--tRNA ligase
  • length: 553
  • theoretical pI: 4.86205
  • theoretical MW: 62380.9
  • GRAVY: -0.425316

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.1.1.19?  ExPASy
    Arginine--tRNA ligase ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
  • ⊞TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 511.7)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 42.6)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 41.6)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 37.1)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 33.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 31.4)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 22.7)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 20.7)
  • TheSEED: see NWMN_0579
  • ⊞PFAM:
    DALR (CL0258) DALR_1; DALR anticodon binding domain (PF05746; HMM-score: 126.6)
    and 12 more
    no clan defined Arg_tRNA_synt_N; Arginyl tRNA synthetase N terminal domain (PF03485; HMM-score: 89.7)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 89.4)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 36.6)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 35.9)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 30.3)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 26.1)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 16.2)
    tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 15.8)
    no clan defined AbiJ_NTD5; AbiJ N-terminal domain 5 (PF18865; HMM-score: 12.9)
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 12.5)
    RBM_T3SS-Flag (CL0873) Spore_III_AF; Stage III sporulation protein AF (Spore_III_AF) (PF09581; HMM-score: 12.3)
    SH3 (CL0010) MTR4_beta-barrel; Mtr4-like, beta-barrel domain (PF13234; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9995
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0004
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006584
    • TAT(Tat/SPI): 0.000488
    • LIPO(Sec/SPII): 0.001017
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDYATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAIIPEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYNVTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHPEIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAKMKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILIDLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLREIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAKEQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFHKFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • ⊟interaction partners:
    NWMN_RS07455dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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