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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03350 [old locus tag: SA0581 ]
  • pan locus tag?: SAUPAN002497000
  • symbol: SA_RS03350
  • pan gene symbol?: mnhD2
  • synonym:
  • product: cation:proton antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03350 [old locus tag: SA0581 ]
  • symbol: SA_RS03350
  • product: cation:proton antiporter
  • replicon: chromosome
  • strand: +
  • coordinates: 673120..674616
  • length: 1497
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (673120..674616) NCBI
  • BioCyc: SA_RS03350 BioCyc
  • MicrobesOnline: see SA0581

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTT
    GTATTTTTAAAAAATAATGATCGTATTTCTAAATATTTATACTTAGGTACAATGACTATC
    ACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGCGTCACCGTCCAATTACGCTA
    GACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTG
    ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGT
    GGCGAACATAAAGCAAATCGTTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGC
    GTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTATACGTCATGTTTGAAATTATG
    TTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
    ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTA
    CTTTATAAAACAGTAGGTACATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATG
    GGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCTTAGTCGCATTTAGTGCGAAA
    GCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
    GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTC
    ACTTTACTATTTGATCAACATAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCT
    ATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATAAAGATATTAAAAAGATTGCA
    GCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
    GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTA
    TTCTTTATTATTGGTAGTTTAGTTTACATTACAGGCTATCGACAATATCAATATTTGAAT
    GGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTATTATAATGATTTTTGCTATT
    GGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
    CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTAC
    AGTTTATTTAGGATATTTTTTTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAAT
    TTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTATTTTAGTAGTTGTGGTTATC
    GCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
    ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03350 [old locus tag: SA0581 ]
  • symbol: SA_RS03350
  • description: cation:proton antiporter
  • length: 498
  • theoretical pI: 9.6341
  • theoretical MW: 55186.9
  • GRAVY: 0.920482

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent K+:H+antiporter (TIGR00944; HMM-score: 366.7)
    and 5 more
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 203.2)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 175.1)
    Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 130.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 93.8)
    NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 67.7)
  • TheSEED: see SA0581
  • ⊞PFAM:
    ComplexI-N (CL0425) Proton_antipo_M; NADH:quinone oxidoreductase/Mrp antiporter, TM (PF00361; HMM-score: 190.5)
    and 2 more
    no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 15)
    DUF1490; Protein of unknown function (DUF1490) (PF07371; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.9994
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0005
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00738
    • TAT(Tat/SPI): 0.000676
    • LIPO(Sec/SPII): 0.039209
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAAIIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGALQNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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