PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA_RS12700 [old locus tag: SA2213 ]
pan locus tag^?: SAUPAN005965000
symbol: SA_RS12700
pan gene symbol^?: bioB
• synonym:
• product: biotin synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12700 [old locus tag: SA2213 ]
• symbol: SA_RS12700
• product: biotin synthase
• replicon: chromosome
• strand: -
• coordinates: 2483674..2484681
• length: 1008
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2483674..2484681) NCBI
• BioCyc: SA_RS12700 BioCyc
• MicrobesOnline: see SA2213

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
  ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
  AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
  TGTCCTGAGAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
  GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
  GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
  AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
  GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
  CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
  GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
  ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
  ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
  AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
  AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
  CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
  CAACCGAACCAATTAGACTACGATATGATTAATGATTTGGGCTTTGAAATTGATTATGAC
  ACTTGTGAAAATAAGGAAAACAAGAATGATGTTTCAAGAGCAAATTGA
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⊟Protein

⊟General

• locus tag: SA_RS12700 [old locus tag: SA2213 ]
• symbol: SA_RS12700
• description: biotin synthase
• length: 335
• theoretical pI: 6.77245
• theoretical MW: 37544.7
• GRAVY: -0.444179

⊟Function

• ⊞reaction:
  EC 2.8.1.6?  ExPASy
  Biotin synthase Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
• ⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.8)
  and 17 more
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 85.4)
  Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 55.1)
  Genetic information processing Protein fate Protein modification and repair 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
  Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate lipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 41.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 35)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 31)
  Hypothetical proteins Conserved radical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
  Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.3)
  radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.9)
  magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 13.1)
  His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.5)
• TheSEED: see SA2213
• PFAM:
  no clan defined BATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 91.2)
  TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 83.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: [2Fe-2S] cluster, [4Fe-4S] cluster
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8188
  • Cytoplasmic Membrane Score: 0.1216
  • Cell wall & surface Score: 0.001
  • Extracellular Score: 0.0585
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.025518
  • TAT(Tat/SPI): 0.002236
  • LIPO(Sec/SPII): 0.001056
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446969389 NCBI
• RefSeq: WP_001046645 NCBI
• UniProt: see SA2213

⊟Protein sequence

• MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRAN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BirA* see SA2213

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications